Ill 
8o° F. Prepare specimens of the glands, as described 
above. Press with the point a needle on the coverglass, 
so that the gland is ruptured, and the secretion poured 
out as droplets into the surrounding fluid. 
Examine with one-sixth inch. If sporozoits are 
present they axe generally very numerous, and large 
numbers of fine, very distinct curved rods, will be 
easily seen with this power, lying throughout the fluid 
around the gland and packed in large numbers in the 
substance of the gland. Finally, examine with one- 
twelfth inch (Fig. 45). 
The sporozoits have a mean length of 14/*,, and 
vary between 10 p and 20^, and are 1-2jl in width. 
Examination of Motion of Sporozoits. —Dissect out 
the glands and, when isolated cleanly, transfer to a 
drop of human serum, previously got ready by allowing 
blood to clot in a small tube. Three kinds of motion 
may be observed :— 
1. Formation of curves. 
2. Formation of ring-formed contractions. 
3. Locomotion. Forward motion. 
Penetration of Red Cell by Sporozoits. —This has 
not been seen in case of sporozoits of the salivary glands, 
but has been observed twice by Schaudinn in the case 
of sporozoits from a ruptured cyst in the stomach. 
Repeat the observation by mixing a little blood with 
sporozoits under a coverglass. 
To Prepare Permanent Preparations of Sporozoits .— 
Pressing firmly upon the coverglass, draw it along the 
slide, so that a film is made on coverglass and slide. 
Dry by rapidly waving the slide and the coverglass 
in the air. Fix both in alcohol, and stain with Roman- 
owsky. Wash, dry, and examine without coverglass 
with an oil immersion, 
The sporozoits appear as fusiform bodies with a 
