3. Sporozoa . — (#) Sausage - shaped bodies in 
masses, sometimes in close connexion with the salivary 
glands (Fig. 48). 
( b ) Octosporozoa, consisting of eight small 
sausage-shaped bodies in small cysts, enormous numbers 
of which replace the yelk of the ovum (Fig. 48). 
(r) Gregarines. —Occasionally on the outside of 
the stomach or encysted in the malpighian tubes. In 
the larvae, the worm-like gregarine will be found 
actively motile in the malpighian tubules. 
4. Flagellates .—In the rectum and hind-gut, 
frequently in enormous numbers, and shewing, when 
stained, large numbers of developmental forms (p. 362). 
5. Developing Embryos of Filaria .—These are 
seen as sausage-shaped bodies with a terminal spike 
in the dissection of the salivary glands. In sections 
of mosquitoes they are seen in the muscles, especially 
in those of the thorax, and the developed embryos 
occur in the labium or about the base of the neck. 
6. Micro-organisms and Sporozoa (Nosema ) ?— 
In the oesophageal diverticula. 
To Cut Sections of Mosquitoes 
The following combined celloidin-paraffin method 
gives admirable results where it is required to preserve 
intact, especially the chitinous parts , and by this method 
and hardly any other it is possible to procure sections 
of a hard proboscis like that, for instance, of Stomoxys 
calcitrans. 
A difficulty that occurs with chitinous organisms 
like flies, is the penetration of the fixative. So difficult 
is it to get perfect fixation that it is advisable where 
details of structure are being studied to remove the 
part required and to fix by itself. 
I. Select Anophelines free from blood and kill 
