201 
3. When the larvae are hatched, transfer them 
(after a day or two) to a larger vessel of fresh water 
containing some weed. When the fresh natural 
appearance of the water disappears, more fresh water 
from a pool should be added. 
4. By keeping larvae in a not too porous earthen¬ 
ware vessel, they may be placed with impunity all day 
in the direct sun. It is necessary, however, to watch 
carefully, to guard against desiccation and consequent 
death of the larvae. 
Larvae kept in flat, partially glazed earthenware 
vessels, with a certain amount of mud, and placed in the 
sun, develop more quickly than those kept in bottles. 
It is of course necessary to make certain that 
foreign ova or young larvae are not introduced with 
the fresh water. 
Some larvae are exceedingly difficult to rear 
artificially, notably those of M. barbirostris and 
M. nigerrimus. They remain for long periods with¬ 
out perceptibly increasing in size, and frequently die. 
(B) An alternative and less tedious way is to 
examine nearly-adult larvae found in nature, and to 
observe, after accurately noting the larval characters, 
what genera and species of Ano'phelines eventually 
hatches out. 
By examining the larva on a slide with or without 
a coverglass, the main characters may be noted without 
in any way damaging the larva, which later becomes 
a nymph and eventually an imago. 
The characteristics of the larvae which are of 
specific importance are, as we have seen— 
1. The antennae. 
2. The clypeal hairs. 
3. The leaflets of the palmate hairs. 
4. The segments carrying palmate hairs. 
