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(ii) In fresh, specimens the female gamete is 
finely granular, and the pigment is frequently scattered 
throughout. On staining, a small amount of chromatin 
is shewn, while the protoplasm takes on a deep blue 
colour. 
Flagellation. —Select an infected bird that shews 
numerous gametes in each field. Proceed in the same 
way as in Proteosoma. The gametes first become 
spherical and then escape from the red cell. The 
pigment of the male gamete displays violent movement, 
and in a few minutes four to eight flagella are extruded. 
The motion of these is at first so rapid that they 
cannot be distinguished, but the corpuscles in the 
neighbourhood are seen moving. In a few minutes 
one or more breaks off, and if, fortunately, a female 
gamete is in the same field, the loose flagellum (mikro- 
gamete) can be seen entering the female. The 
pigment of the latter shews active movements at this 
stage. 
Vermiculi. —The formation can readily be 
observed on the slide. A conical projection forms at 
one point of the fertilized gamete (copula). This 
elongates slowly and gets curved, forming an egg- 
shaped or spindle-shaped mass. The conical portion 
eventually separates, leaving behind the remains of 
the cell with the pigment. The vermiculus is thus 
at first unpigmented, but later again it is pigmented 
(Koch). In the fresh specimen the protoplasm appears 
vacuolated, and has a nucleus which is readily stained 
by Romanowsky stain. 
Note that the vermiculus (or ookinet) shews 
forward, rotatory, and peristaltic motions. 
Development in the Fly. —The brothers Sergent 
have shewn that infection in the case of H. columhae 
of the pigeon is transmitted by a Hippoboscid, Lynchia 
