290 
containing each i c.c. of normal salt solution. When 
parasites are abundant in the blood, chloroform a dog 
and take blood from the heart. Defibrinate by 
shaking up in a sterile bottle with pieces of glass, 
wire, etc. To each tube of salt solution add i c.c. of 
defibrinated blood. Keep at about 27 0 . Examine 
next day. Stellate forms are described by Kleine 
similar to those seen by Koch in ticks (p. 281). These 
subsequently become spherical. Fulleborn, on the 
contrary, describes flagellates in culture. 
7. P. ovis .—Occurs in Europe, Africa and West 
Indies. The disease is characterised by (1) Intense 
anaemia, the number of blood cells falling from eight 
to one million ; (2) Haemoglobinuria, the urine 
contains also red cells and bile pigment ; (3) Bloody 
diarrhoea ; (4) A mortality of about 50 per cent. 
On the other hand the symptoms may be exceedingly 
slight. 
Blood Examination. —Fairly large intracorpuscular 
and extracorpuscular forms occur. 
Post-mortem. —The tissues are very oedematous. 
The spleen is enlarged. The liver especially, and the 
kidneys shew marked inflammatory changes. The 
gut is inflamed and ulcerated. 
Transmission. —(1) By inoculation ; (2) Eu. bursa 
is the carrier in Hungary. As in P. canis , it is only 
the adult tick, the daughter of an infected mother, 
that conveys the disease. The incubation period is 
about seven days. It is uncertain whether the blood of 
recovered sheep is infective. 
Eu. bursa 
Adults take infection from a sick sheep. Drop on to 
the ground and lay eggs which develop into 
