3*4 
The Male .—Treat as in the case of the ungorged 
female. Note the distended vasa deferentia and the 
narrow alimentary diverticula (Fig. ioi). 
Phe Nymph .—To prepare specimens of the freshly 
gorged nymph cut off, by pressing with a sharp scalpel, 
a thin slice from the posterior end, and passing the 
scalpel from before backwards, press out the whole of 
the contents into a drop of normal saline. Search for 
separate organs with a low power lens. To obtain 
specimens of the tissues of nymphs which have advanced 
towards metamorphosis, treat as in the case of the 
ungorged female, or better, cut off the anterior one- 
third of the body by means of a sharp scalpel and 
turn out its contents. Observe that a rough model 
of the future adult in embryonic tissue is present. 
For observing developmental stages of Piroplasma , 
make smear preparations of this tissue and look under 
a low power for parts where salivary ducts and young 
acini are forming. 
Examination of Ticks 
Place a tick alive upon a slide and cover with a 
second slide. Pass a rubber band over each end (or 
tie with thread) so as to compress the tick into an 
extended position suitable for examination. (Rubber 
bands are easily made from a piece of rubber tubing). 
In this way note the characteristics of ( a) the unfed 
larva ; ( h ) the gorged larva ; ( c) the unfed nymph ; 
(d) the gorged nymph ; ( e ) the unfed female adult ; 
(/) the unfed male adult; ( g ) the gorged female adult 
(Fig. 94). 
Po Mount Picks — 
Method I. (a) Place the ticks mounted as above 
