3i5 
(slides, rubber bands and all) into absolute alcohol for 
about an hour. 
(b) Remove the rubber bands and place the 
tick in (CuS 0 4 ) absolute alcohol for about another 
hour. 
Clear in oil of cloves and lower into a large drop 
of thickish balsam so as to avoid air bubbles. 
Method II. Kill bp placing in boiling water, 
then proceed as in Methods I, III, etc. The tick is 
suitably extended by this method. 
Method III. {a) Boil the compressed tick for 
from ten to thirty minutes, or even much longer, in 
ten per cent, caustic alkali, until it looks quite trans¬ 
parent. 
In the case of soft bodied ticks, especially gorged 
nymphs and larvae, it is necessary to regulate the 
pressure, or they may become ruptured either before 
or after placing them in the solution. For nymphs and 
larvae coverglasses may be used in place of slides. 
(b) Remove the tick and wash thoroughly in 
water till quite free from alkali. 
(c) Stain in a saturated solution of fuchsin (if 
desired), wash in alcohol. 
\d) Dehydrate, clear and mount in balsam. 
Method IV. For ticks that are already dry, boil 
in ten-per cent, potash or soda till clear. Wash in 
water, dehydrate in alcohol, clear in oil of cloves and 
mount in balsam. (Stain in fuchsin after the boiling, 
if necessary). 
It is often advisable to open the tick or remove 
some of the dorsal integument in order to see the 
mouth parts clearly (Newstead). 
Method V. To Cut Sections of Ticks .—Take ticks, 
as young as possible , best of all those that have only just 
moulted, then proceed as on page 116. 
