336 
the effect. In these rosettes there is no loss of motility, 
and the clusters may separate again. In cultures, e.g., 
of T. lezvisi, the trypanosomes are agglomerated by their 
anterior ends (Fig. 107). 
Fig. 107. (1) Agglomeration of trypanosomes ; (2) Culture forms 
( Herpetomonas ) in rosette 
Cultivation of Trypanosomes 
Novy-MacNeal-Mathis Medium .—1. Collect the 
blood of any suitable animal, e.g., cow, rabbit, etc. 
(strict asepsis is not absolutely necessary). 
2. Defibrinate by shaking up with wire, or glass 
beads, etc. 
3. Add one part of blood to two of agar in a 
number of tubes. 
4. Sterilize once or twice at ioo°C., and make 
slopes. ( Vide also p. 353.) 
5. Inoculate the condensation water with a little 
blood of the trypanosome to be grown, e.g., T. lezvisi, 
and keep at room temperature. 
