353 
blood, in equal parts, or two parts of blood and one 
of agar. 
The blood maybe got by bleeding from the carotid, 
or by inserting a trocar and cannula directly into the 
heart, through the thorax, in an anaesthetized rabbit. 
Collect in a sterile jar, shaking well with pieces of glass 
rod or wire, etc. Mixing the blood and agar in flasks 
or as ordinary ‘ slopes,’ and after covering with rubber 
caps, place in the incubator, at 37 0 , over night, to make 
sure of sterility. {Vide also p. 336). 
Inoculate the tubes with rats’ blood containing 
T. lezvisi , taken from the heart by means of a sterile 
pipette, and incubate at 25 0 C. As soon as a good 
growth (rosettes) is obtained, sub-cultures should be 
made. 
The cultural forms vary in length from 1-2^ to 
60/^. Most characteristic are the rosettes, consisting 
of large masses of trypanosomes with their flagella 
centrally placed. The position of the blepharoplast 
is anterior instead of posterior, and the undulating 
membrane but little developed. The cultures are 
infective, and Novy and MacNeal state that, after 
passing the culture fluid through a Berkefeld filter, the 
filtrate is still infective. 
In Muridae, we have also 7 \ longocaudense in the 
Indian Mus niveiventer , and 7 *. dutto?ii in the Senegal 
mouse Mus mus cuius , and 7 ”. blanch ardi in Myoxus 
nitela , garden dormouse. 7 ". myoxi in M. avellanarius , 
red dormouse. 7 ". bandicotti (? = 7 ". lewisi) in the 
bandicoot Nesokia gigantea. 
Trypanosomes in Bats 
(1) 7 ". nicolleorum in Vesfertilio kuhli and Myotis 
murinus in Africa. A small (24/^) active form and a 
large (30/*) sluggish form are described, 
z 
