364 
matopota italica. It is 30 by 1-2/q flagellum 20-25/A. 
(Fig iii). 
Dissect out the gut of a mosquito, e.g., C. fatigans. 
Note with the naked eye (in an infected mosquito), the 
distension of the gut by the flagellates. Rupture the 
gut and make smears. Stain with Romanowsky. 
Significance of Gut Flagellates. —In the case of 
F. gambiense , we know that all traces of trypanosomes 
disappear from the gut of the fly in ninety-six hours 
(but it is quite possible that they still exist in some 
unknown form). 
Similarly, in the case of F. brucei , the trypanosomes 
disappear in fifty-five hours. On the other hand, in 
the case of fish trypanosomes, multiplication occurs in 
the leech, and they are infective for weeks. 
It is necessary, therefore, to bear in mind the 
existence of these flagellates in the gut, sometimes in 
enormous numbers, especially as the cultural form of 
a trypanosome is of a herpetomonas type, i.e ., spindle 
shape, with anterior blepharoplast. 
Pure cultures of gut flagellates are best got on 
blood-agar plates by a series of diluting smears. 
Genus Trypanoplasma 
Anteriorly has a long rod-like blepharoplast. 
Close beside this are two minute basal bodies, from 
which the flagella arise; the anterior free flagellum is 
attached anteriorly to one basal body, while the 
posterior, arising from the other, is attached along 
the whole length to the edge of the undulating mem¬ 
brane, and then terminates posteriorly in a free 
flagellum (Fig. 112). 
Half a dozen of these flagellates are already known 
in the blood of fishes. They vary from 12-40/* in length. 
