3^9 
3- Further observe that polynuclear leucocytes 
contain only one or two of these bodies (Fig. 16); 
large mononuclear leucocytes one to six (Fig. 17) ; 
cells of an endothelial type one to twelve (Fig. 18) ; 
large cells with a hyaline or finely granular or vacuo¬ 
lated cytoplasm (macrophages) up to several hundreds 
(Fig. 19). 
4. The parasites are approximately circular or 
oval, 2*5-3*5 M ' m s i ze > clesLily outlined, and appear 
to possess a distinct cuticle, as they retain their shape 
and are rarely seen distorted in films. 
5. The two chromatin masses are characteristic, 
the large one staining lightly and the small one intensely 
with Romanowsky. The masses are usually situate 
opposite each other in the short axis of the parasite. 
The larger chromatin mass always forms part of the 
periphery of the parasite. 
6. Most of the parasites contain one or two 
vacuoles which may displace, the cytoplasm of the 
parasites to the periphery. 
7. Developmental forms. Division commences 
at the thick end of the parasite, and the large chromatin 
masses may be widely separated before the small 
chromatin mass has begun to divide. As many as three 
to six bodies are formed in this way, the large nuclei 
being arranged peripherally, and the smaller centrally 
(Figs. 7-11). 
Occurrence in Peripheral Blood 
Parasites can be found in the blood in about 
eighty per cent., if not in all cases. Properly spread 
films (p. 19) must be used, especially as leucopenia is 
so common, and the leucocytes carefully examined ; 
for the parasites do not occur free. It may be necessary 
to examine more than one film (Patton). 
AA 
