373 
Lymphatic glands .—In those draining the area of a skin lesion 
parasites are found. They occur in large cells in the lymph sinuses 
and in cells of the reticulum. 
Development .—Patton Jias traced the development 
of the parasite in the Indian bed bug Cimex macro- 
cephalus up to the Herpetomonas stage, as seen in 
cultures. 
Culture .—Add blood taken bp a splenic puncture 
to a drop or two of ten per cent, sodium citrate in 
a small test tube. Slight acidification with citric acid 
Fig. 113.—(1) Parasite as seen in splenic blood; (2) Third 
day , parasite enlarged ; (3) Fourth day , full size ; 
(4) Chromatin masses at the extremities; 
(5) Stumpy flagellate forms; (6) Elongate flagellate forms 
assists development (Rogers). Incubate at 22 0 to 24°C. 
About the third dap the parasites are as big as a red cell 
and the nuclei are no longer peripheral (Fig. 113). 
These forms map divide, forming groups, or flagella 
map develop directlp from the enlarged parasite. 
About the fourth to sixth dap elongate flagellates or 
forms still stumpp appear. Thep resemble Herpeto¬ 
monas forms. Leishman describes also ‘ spirochaete ? 
forms. 
