4 °5 
4. Acetic Alcohol Sublimate .— 
Absolute alcohol - - 1 part. 
Glacial acetic acid - - 1 part. 
Chloroform 1 part. 
Sublimate, to saturation. 
An excellent fixative for general purposes. Fix 
for one hour or several, according to size of tissue. 
Wash out the acid in alcohol. 
5. Formalin (forty per cent, solution of formal¬ 
dehyde).—Use ten per cent, solution in water or normal 
salt. Small pieces are fixed in an hour or so. Transfer 
to alcohol. 
6. Alcohol .—Is a fixative and dehydrating medium, 
and should only be used alone in an emergency. 
The tissues, in small pieces, may be placed directly in 
methylated spirit (ninety per cent, alcohol), or absolute 
alcohol (ninety-eight per cent, alcohol). Change the 
alcohol a few times. After hardening, if the specimens 
are not to be imbedded immediately, transfer to 
alcohol of about eighty per cent, for preserving. 
Rectified spirit of the British Pharmacopoeia, 
is equal to eighty-four per cent, alcohol. 
Methylated spirit, containing wood naphtha, is 
equal to ninety per cent, alcohol. 
Ordinary methylated spirit contains mineral 
naphtha, and should not be used. 
For practical purposes the dilution of alcohols is 
sufficiently accurately made by means of the diluting 
formula (p. 411). 
7. ZenkeFs Fluid .—■ 
Potassium bichromate - 2*5 grammes. 
Sodium sulphate - - ro gramme. 
Corrosive sublimate - 5*0 grammes. 
Water - ioo*o grammes. 
This is the stock solution. When about to use, 
