408 
2. Haematein .— 
Haematein {puriss. ) 0*4 gr. 
Alum ----- 5*o gr. 
Glycerin - 30*0 c.c. 
Water ----- 70*0 c.c. 
Rnb up the haematein with a little glycerin, and 
add the other ingredients. The stain is ready for use 
at once, and keeps well. 
3. Methylene Blue. —For staining blood or 
tissues, use a quarter per cent, watery solution of pure 
methylene blue. 
4. Eosin. — This is really Brom-eosin, i.e., 
yellowish or water-soluble eosin. For blood work and 
also for tissues is best used in the form of a half per cent, 
solution in seventy per cent, alcohol. 
Eosin* - - - - o*5 grm. 
Absolute alcohol - - - 7o # o c.c. 
Water ----- 30*0 c.c. 
5. Ehrlich's Eriacid. — Take Et 2 0 , 45 c.c. ; 
glycerine, 10 c.c. ; alcohol, ninety per cent., 25 c.c. ; 
and add in the following order : acid fuchsin, 3 grm. ; 
orange G, 2 grm. ; methyl green, 1 grm. Stains 
typically and keeps well. 
Iron Reaction ( Haemosiderin) in Malarial Eissues .— 
(1) Treat the sections after removing the paraffin 
with two per cent, watery solution of potassium ferro- 
cyanide for from five to twenty minutes. 
(2) Acid alcohol (HC 1 1 part, seventy per cent, 
alcohol 100 parts) five to ten minutes. 
(3) Wash in water. 
(4) Counterstain with alum carmine. 
Eo Mount Specimens in a Fluid Medium. —Remove 
all fluid from the edge of the coverglass. Seal with 
plenty of melted glycerine jelly. Examine next day to 
* Use ‘ pure French ’ eosin or a spirit-soluble eosin. 
v\ 
