# Cells /mb 
Days 
r e 
C u 
1 .2x10 
' 5 tyl 
1 .2x10 6 M 
Contro 1 
□ 
o 
4 x IO~ 8 M 
A 
1.2 x IO' 7 M 
• 
▲ 
4.4 x ICT 7 M 
■ 
▼ 
Expt. #1 Expt.#2 Expt.#3 
-Experiments #1,2,3 done with inocula from 
different cul tures 
-No EDTA 
Figure 4-7. Antagonistic effects of iron and copper on 
the growth rate of Pyramimonas 1 in artificial seawater medium 
with the usual supplements of F/2 medium except for EDTA 
which is not added. 
Note: Experiments 1, 2, and 3 represent inocula from different cultures. 
"Fresh" iron is FeCl 3 solution prepared the day of the experiment and 
sterilized by filtration. 
52 
