INFLOW 
Figure 10-1. Diagram of Flow-through Oil Dosing System. 
NOTE: See text for details. 
different postures and movements) the times for alert (first observable response 
to food), wait (period between alert and leaving shelter to search), and search 
(time after first leaving shelter until food touched with maxillipeds) were 
recorded. On the morning of the 6 th day, #2 fuel oil was introduced at a 
predetermined flow rate. Lobster behavior was recorded 6 hours later and 
subsequently twice daily, as above. After five days, oil introduction was 
stopped. All recordings were continued as before for another five days to 
determine behavioral and chemical recovery rates. 
Oil Chemistry 
Concentrations of total hydrocarbons in the water column were determined 
by infrared spectroscopy before, during, and after introduction of oil. On the 
day before oil was added, a 2-1 water sample from every tank was extracted 
with 50 ml CClq. A second extraction was performed 12 hours and a third 24 
hours after onset of oil mixing. Extractions were continued daily for the 
remaining four days of oil exposure, and on the first and second day 
post-exposure to determine how quickly oil left our system. A small number of 
CH 0 CI 9 extractions were performed for gas chromatographic analysis. The oil 
used in the study was an Exxon #2, provided by the EPA Environmental 
Research Laboratory, Narragansett, Rhode Island. 
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