Table 15-2. Composition of the Artificial Diets, 
CM-1 and C-1 
Components 
Percent Composition 
CM-1 
C-1 
Herring meal 
40.00 
10.00 
Soy bean oil 
10.00 
10.00 
Corn gluten meal, 60% 
10.00 
10.00 
Wheat middlings, standard 
9.00 
9.00 
Brewers dried yeast 
5.00 
5.00 
Dried condensed fish solubles 
5.00 
5.00 
Dried Whey 
5.00 
5.00 
Meat and Bone Meal 
5.00 
5.00 
Soy bean oil 
— 
10.00 
Cod liver oil 
i 
Mineral mixture 
10.00 
— 
0.40 
0.40 
Vitamin mixture 
0.70 
0.70 
Mixture provided the following compounds in g/kg diet: MgSO^, 2.0; ZnSO^-l-^O, 0.3; 
FeS0 4 -7H 2 0, 0.3; CuS0 4# 0.3; KIO^ 0.0091 and MnSC> 4 -H 2 0, 1.0. 
Mixture provided 10,000 IU Vitamin A as retinyl palmitate; 4,000 IU Vitamin D as 
Cholecalciferol; 75 IU Vitamin E as dl-a-tocopheryl acetate; and the following amounts 
(milligrams) of other vitamins per kilogram of diet: menadione dimethylpyriminidol 
bisulfate, 10.0; thiamine HCL, 4.0; riboflavin, 30.0; calcium pantothenate, 150.0; 
niacinamide, 300.0; pyridoxine-HCL, 20.0; d-biotin, 6.0; folacin, 15.0; Vitamin B.^, 
0.002; L-ascorbic acid, 1000; inositol, 500.0; butylated hydroxytoluene (100%), 100.0; 
and choline chloride (70%), 1330.0. 
methods of Spackman et al (31) and Moore and Stein (27) with modifications 
by Niederwieser and Pataki (29), Blackburn (10) and Hirs (21). Amino acid 
analyses were performed on a Technicon Auto Analyzer (NC-2P) with a 25 cm 
column. An electronic integrator (Columbia Scientific Supergrator 2) was used 
to compute the absolute amounts of each amino acid. 
Protein content was assayed by microkjeldahl according to Hiller et al (20), 
and the moisture content was determined using procedures described by 
Chibnall et al (13). 
218 
