Table 15-3. Composition of the Artificial Diets, 
CMP-1 and CMP-2 
Composition 
Percent Composition 
CMP-1 
CMP-2 
Casein 
40.00 
40.00 
Gelatin 
10.00 
10.00 
Cod liver oil 
10.00 
10.00 
Sucrose 
10.00 
10.00 
Dextrin, white Technical 
i 
Cellulose 
10.00 
10.00 
4.26 
7.16 
Choline chloride (70%) 
0.30 
0.30 
L-glutamic-HCI 
1.20 
— 
L-ascorbic acid 
0.30 
0.30 
L-glutamic acid 
6.30 
— 
NaCI 
0.50 
0.50 
Mineral mixture^ 
6.40 
6.40 
Vitamin mixture 
0.50 
0.50 
DL-Methionine 
0.20 
0.20 
L-tryptophan 
0.04 
0.04 
Amino Acid mixture^ 
— 
4.60 
Solka floe. Brown Company, Berlin, N.H. 
Minerals in g/kg diet: CaHP0 4 H 2 0, 18.04; CaC0 3 , 19.04; KH 2 P0 4 , 14.03; NaHC0 3 , 
8.82; MnS0 4 H 2 0, 0.35; FeS0 4 H 2 0, 0.50; MgSO 4 ,3.02; KI0 3 , 0.01; CuS0 4 -H 2 0, 
0.03; ZnC0 3 , 0.15; CsC1 2 -6H 2 0, 0.002; NaMo0 4 H 2 0, 0.008; and Na 2 Se0 3 , 0.002. 
The vitamin mixture included 10,000 IU Vitamin A as retinyl palmitate; 4,000 IU 
Vitamin D as Cholecalciferol; 75 IU Vitamin E as dl-a-tocopheryl acetate; and the 
following amounts of vitamins in mg/kg diet: thiamin'HCL, 40.0; menadione 
dimethylpyrimidinol bisulfite (Vitamin K), 2.0; riboflavin, 30.0; D-calcium 
pantothenate, 150.0; niacin, 300.0; pyridoxine'HCL, 20.0; d-biotin, 0.5; folic acid, 
15.0; Vitamin B^, 0.3; Ethoxyquin (100%), 200.0; and myo-inositol, 500.0. 
Amino acids in g/kg diet: L-threonine, 7.0; L-valine, 5.0; L-cystine, 3.0; L-lsoleucine, 
10.0; L-leucine, 8.0; lysine'HCL, 2.0; and L-arginine'HCL, 11.0. 
Lipid Extraction and Analysis 
Samples were collected, weighted, measured, lyophilized, and stored at 
-20°C under nitrogen. Several small fish or approximately one gram of each 
diet for each sample were rehydrated with 5 ml distilled water. Samples were 
extracted twice in a Sorvall Omni-mixer (60 ml capacity), according to the 
Bligh and Dyer (11) technique as modifed by Kates (23). Lipids were 
219 
