the indophenol technique of Solorzano (34). Salinity measurements were made 
using an American Optical salinity refractometer. With frequent water changes, 
most of the water quality parameters changed insignificantly during the course 
of each experiment. In later experiments where the number of individual 
treatments became unmanageable, regular monitoring of all variables except 
temperature was discontinued, and a stringently maintained schedule of water 
changes observed. 
Feeding larvae were supplied with newly hatched Artemia nauplii at least 
twice a day in quantities sufficient to permit a portion to remain until the next 
feeding. Artemia nauplii proved to be a satisfactory diet for striped bass 
through the early juvenile stage. 
Larval growth was measured in terms of dry weight. Prior to weighing, 
larvae were lifted on a No. 6 sable brush, dipped in distilled water to remove 
any adherent salt or debris, blotted on filter paper and placed on a tared 
weighing pan. Pans were cut out of aluminum foil with a paper punch and 
ashed 4 hours at 500°C before use. Dry weight determinations were made after 
the specimen on its tared pan had been dried to a constant weight in a heated 
vacuum desiccator at 80°C over silica-gel. On larvae up to metamorphosis, 
weights were determined using a Cahn “Gram” electrobalance. Weights were 
read to the nearest microgram. 
Design of Experiments 
Temperature-delayed feeding-survival experiments were performed during 
the springs of 1976 and 1977. A total of three experiments were performed, 
each identical in its design. In each, five containers were placed in each of four 
constant temperature water baths. Each container was stocked with 100 
prolarvae at the temperature at which they had been held prior to the 
beginning of the experiment. Stocked containers were assigned to particular 
temperature treatments by lot. The acclimation period from holding 
temperature to the experimental temperature treatment was at most one hour. 
In each temperature treatment, the larvae in one container were offered a 
diet of newly hatched live Artemia nauplii at the beginning of the experiment. 
Food was withheld from another container throughout the observation period. 
The time of first feeding for larvae in each of the remaining three containers of 
starved larvae in each temperature treatment was determined on the basis of 
observations of the apparent state of health of individuals in each population. 
Each container was checked for mortality several times each day throughout 
each experiment, and all dead larvae removed. 
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