24 BULLETIN 740, U. S. DEPARTMENT OF AGRICULTURE. 
giving off the water, which forms a layer in the upper part of the 
container). 
10. Interrupted washing (draining and measuring during washing) 
results in a smaller osmotic increase than does continuous washing 
for the same length of time. * , 
11. The relation of osmotic loss to actual loss varies with the solu¬ 
bility of the substance in question. Thus, in the average of all the 
experiments, in the case of total solids made up of insoluble and 
soluble constituents, the ratio of osmotic loss to actual loss is approxi¬ 
mately 2:1; for amino-acid nitrogen, all of which is soluble, but 
which goes into solution slowly, the ratio is approximately 1:1; and 
for ash-forming substance, which is composed largely of soluble 
inorganic compounds, the ratio is approximately 1:2. 
I 
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