IN THE SEED WHICH ACCOMPANY GERMINATION. 
45 
24 hours. This treatment removed any crystalline bodies present in the extract 
used, leaving in the final dialysate only such as had been formed in the latter part of 
the digestion. The quantity found in the last ] 20 c.c. was as great as in the former 
experiment. On watching from time to time the progress of the action, as shown by 
the changes in the fibrin, a correspondence was evident between the behaviour of the 
lupin extract and that of pancreatic juice. True, the reaction of the fluid was 
different, but the fibrin seemed to be eaten away from the outside just as in 
pancreatic digestion, and not to gradually pass into solution, as it does when acted on 
by pepsin. When the digestion was complete the liquid was quite turbid, and 
deposited, on standing, a sediment of fine particles. In the tubes in which the fibrin 
was only treated with HC1 it maintained throughout the peculiar, almost translucent, 
appearance characteristic of it almost immediately it is subjected to the acid’s action, 
and the liquid was only slightly opalescent after days of treatment. 
In no case during the experiments did bacteria appear in any of the tubes. 
The course of digestion as estimated by the products formed also closely resembled 
that brought about by trypsin. In the process caused by the latter there are three 
distinctive bodies or groups of bodies formed apparently successively. The first of 
these is precipitated on neutralising the digesting mixture, and the precipitate is 
soluble in either weak acids or alkalis. To it has been given the name of parapeptone. 
Very soon after digestion has begun, the so-called peptone is recognisable, and leucin 
and tyrosin, crystalline bodies, appear. Besides these, there are formed, simultaneously 
apparently with the parapeptone, bodies called albumoses, which possess the peculiar 
properties of being insoluble, some in slightly acid solutions, some in water, at 
ordinary temperatures, but being soluble at temperatures higher than 70° C. In the 
digestions by the lupin extract the liquid in the dialyser very soon gave a conspicuous 
neutralisation precipitate, soluble in acids or alkalis, and being evidently parapeptone. 
Besides this there appeared to be a considerable amount of albumoses formed, more 
than is usually seen with either pepsin or trypsin. These were precipitated with the 
parapeptone, but could be readily separated from the latter by warming the tube 
containing the mixed precipitates suspended in water. A good deal of the suspended 
matter dissolved, and, on filtering while hot, the insoluble parapeptone was removed, 
while the albumose remained in solution and was thrown down again as the liquid 
cooled. The albumose in greatest quantity here differed somewhat from the hemi- 
alburaose of Kuhne, or a-peptone of Meissner, as this body is precipitated by acids. It 
corresponds more closely with Kuhne’s heteroalbumose,' 1 ' which is insoluble in water, 
being thrown down by dialysis. A certain amount of clysalbiunose also was present. 
According to Kuhne, the albumoses are partly the result of the action of the acids 
on proteids, for they are formed by digesting fibrin with HC1 - 2 per cent, for a con¬ 
siderable time. In this case they were not so formed, but were due to the action of 
the extract, for a control tube with IICl only and fibrin contained a mere trace of them. 
* Kuhne and Chittenden, ‘Ueber Albumosen,’ ‘ Zeitscbr. Biol.,’ vol. 20, 1884, p. 11. 
