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MRS. G. C. FRANKLAKD AND DR. P. F. FRANKLAND 
expansion consisting of small hump-like protuberances. In nine days (August 6—15, 
1886) the surface-growth was still very restricted, but had formed a depression filled 
with lemon-yellow semi-liquid matter. Even after eighteen days (August 6—24) 
there was but little change in the needle-track, but the surface-depression, which was 
considerable (see Plate 18, No. 6a), was filled with liquid, at the bottom of which 
was a lemon-yellow deposit. 
This organism thus causes a very slow liquefaction of the gelatine, and produces a 
very decided lemon-yellow pigment. 
Aqar-agar. —Forms a thick chrome-yellow moist mass extending over the surface. 
Broth. —After nine days (August 7—16, 1886) the liquid is clear and free from 
pellicle ; there is a lemon-yellow deposit at the bottom. 
Appearance on plate-cultivation. —The colonies are visible to the naked eye as 
small yellow centres which, under a low power (x 100), appear of irregular shape, 
finely granular near the periphery, the edge being nearly smooth (see Plate 18, 
No. 6c). The centre of the colony has a dark greyish-green colour. No liquefaction 
was observed during the first six days. 
5. Sarcina Aurantiaca. 
Origin. —This organism is also occasionally found in the air, although we have not 
ourselves met with it there. A cultivation was brought by one of us fron Dr. Koch's 
laboratory in Berlin. The only references to this organism which we have been able to 
find are in Eisenberg’s ‘ Bakteriologisclie Diagnostik,’ which contains a very brief 
description of its appearance, and its existence is mentioned by Flugge (‘Die Mikro- 
Organismen ’) without, however, any description being appended. 
Microscopic appearance. —Under the high power (X ],000 or X 1,500) there are 
seen packets of cocci which are much smaller than those of Sarcina lutea. The 
complete packet of four cocci measures only about 1‘7/r across. (See Plate 18, 
fig. 4, No. 46.) 
Appearance in Cultivations. 
Gelatine. —In the tube, after four clays (August 6—10, 1886), liquefaction has 
taken place along the path of the needle, producing a funnel-shaped canal which is 
filled with clear liquid, at the bottom of which is a flocculent orange deposit. 
The Plate 18 (No. 4a) exhibits the condition of the cultivation after seven days’ 
growth (August 6—13) ; the liquefaction even then has not extended across the tube, 
and the lower extremity of the needle-path is still comparatively undeveloped. 
Agar-agar. —Forms an abundant and moist surface-growth of a strong orange 
colour. The growth is for the most part continuous, but numerous little heaps are 
distributed over the remainder of the surface, 
Broth. — After nine days (August 7—16, 1886) the liquid is turbid at the surface, 
