274 
MRS. G. C. FEANKLAND AND DR. P. F. FRANKLAND 
Appearance in Cultivations. 
Gelatine. —The growth to which this organism gives rise in gelatine is exceedingly 
characteristic. On the surface there appears along the needle-streak a much 
crumpled and folded greyish expansion, the peripheral corrugation of which causes the 
surface to become abundantly pitted and excavated. The growth in the needle-track 
below is much less vigorous than on the surface, although in course of time it becomes 
developed to a considerable extent and has a beaded appearance. 
It causes no liquefaction of the gelatine, even in old cultivations. (See Plate 18, 
No. 7a.) 
Agar-agar. — The appearance is very similar to that of the cultivation in gelatine; 
the surface is, however, of a somewhat more moist texture, and the edge extends in 
thin fern-shaped expansions over the surface of the agar-agar. 
Broth. —After nine days (August 7—16, 1886) the liquid is very slightly turbid, 
has a dirty-white deposit, and there is a small amount of flocculent matter on the 
surface ; and, adhering to the sides of the tube, this develops later on into a tough 
irregular pellicle. 
Appearance on plate-cultivation.—Alter four days the colonies appear to the 
naked eye as small white discs, the larger ones, which have reached the surface, 
exhibiting an indentation in the centre. As growth proceeds, the centre of the colony 
remains depressed, whilst the circumference becomes irregularly folded and raised, so 
that the colony is only attached to the surface of the gelatine by a comparatively 
narrow pellicle. The substance of the colony is very tough in character, so that the 
whole growth can be easily removed in its entirety by means of a needle. 
Under a low power (X 100) the small colonies have a rough irregular edge varying 
in shape and degree of roundness. The larger colonies are dark-brown near the edge, 
but of a lighter shade near the centre ; they are very irregular in shape ; the contents 
are finely granular. The different stages of development are exhibited in Plate 18, 
No. 7c. 
5. Bacillus Chlorinus. 
Occurrence. —This was found as a yellow slowly-liquefying expansion on the surface 
of a gelatine dish exposed to the air on the spire of Norwich Cathedral. We have 
found it on numerous occasions to be very prevalent in air. 
Microscopic appearance. —Under a high power this is seen to be a very short 
bacillus, varying from '5/x to 1 ‘5/x in length and about half as broad as long; the 
extremities are rounded. It occurs singly and in short chains. (See Plate 17, 
fig. 7, No. 7b.) In drop-cultivations only vibratory motion was observed. 
