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MRS. G. C. FRANKLAND AND DR. P. F. FRANKLAND 
turbid, free from pellicle, and has a small quantity of white deposit at the bottom. 
Even after thirteen days there is only a thin film on the surface, which falls to the 
bottom on shaking, and there is very little deposit. 
Appearance on plate-cultivation. —After two days the colonies appear to the naked 
eye only as white specks, but seen with a low power (X 100) those on the surface 
exhibit a very irregular contour, consisting of branchings into the surrounding 
gelatine of threads ; the interior of the colony has the appearance of being composed of 
threads closely packed together; as they develop further, the centre becomes very 
dark and cloudy, but the edge remains very light, and thus much resembles a crystal 
branching out in feathers into the surrounding gelatine ; after five days the feathery 
contours can be seen with an ordinary magnifying glass. In the depth the colonies 
appear compact and almost circular. (See Plate 19, No. 7cl.) 
In all cultivations this organism gives rise to a most disagreeable odour, somewhat 
resembling that of putrid blood. 
9. Bacillus L/Evis. 
Occurrence. —This was found forming a yellowish-white liquefying growth on the 
surface of a gelatine-dish which had been exposed to the air in one of the wards of the 
Brompton Hospital for Consumption. 
Microscopic appearance. —Under a high power this is seen to be a bacillus the 
average length of which is 1'7 to 2'5/r, and it is about 5 times as long as broad ; the 
ends are distinctly rounded. It occurs singly, often in pairs, and occasionally in 
threads. It gives rise to spores which are nearly as long as the bacillus itself, but 
more oval in shape, and which exhibit the characteristic highly refractive appearance 
of spores in general. All the well-known forms of Bacillus subtilis were observed, 
including the thickened form, only on a much smaller scale, and the threads being 
considerably shorter. 
In preparations made from the surface of agar-agar cultivations frequently nothing 
but spores were visible. Whilst the bacillus is readily stained with any of the 
ordinary aniline colours (gentian-violet, &c.), the spores prove refractory as usual. 
In Plate 19, fig. 6, No 6c, the preparation was made from a gelatine-cultivation of 
ten days’ age. In No. 6b the appearance is shown when a preparation is made 
from a colony after three days’ growth. Tube-cultivations started from such colonies 
yielded in course of time all the various forms represented in No. 6c. 
In drop-cultivations the bacilli are seen to be exceedingly motile, occurring singly, 
in pairs, and occasionally in threads ; subsequently stationary masses of bacilli make 
their appearance, and shining spores are visible. 
