286 
MRS. G. C. FRANKLAND AND DR. P. F. FRANKLAND 
Under a low power (x 100) the branches of the star are seen to be composed of 
the individual cells closely compressed together in packets. The appearance is shown 
in Plate 19, lc. No le represents the naked-eye appearance of a colony. 
In its microscopic appearance this saccharomyces appears to correspond with the 
description of S. apiculatus, but the behaviour of the latter in cultivations has not 
been described. 
2. Saccharomyces Rosace us. 
We have found this on gelatine-plates which have been exposed to the air. It 
forms small pink pin-head colonies on the surface of the gelatine. 
Microscopic appearance .—The cells are long, oval, about 8 - 5/x long and 3'5/x in 
width. They appear to bud from the extremities only, and there are frequently 
two twin buds adhering to the same extremity of the parent cell. They are 
sometimes seen hanging together in chains of four. (See Plate 19, 2b.) 
Appearance in Cultivations. 
Gelatine .—It forms a shining pink expansion on the surface, there being but little 
growth in the track of the needle below. 
Agar-agar .—On the surface it forms a smooth shining growth of a beautiful 
rose colour. The growth exhibits lines of darker tint radiating from a lighter centre 
(see Plate 19, 2a). There is no colour and but little growth in the needle-track below 
the surface. 
Broth .—After six days the liquid is clear, with a dirty-pink deposit at the bottom, 
which later on becomes of a bright-pink colour, the liquid remaining clear. 
Appearance on plate-cultivation .—The colonies are seen to the naked eye as 
shining pink pin-heads, which do not cause liquefaction of the gelatine. Under 
a lower power (X 100) the individual cells composing the closely packed masses of 
which the colonies consist can be distinctly seen, especially in the case of the surface 
colonies. (See Plate 19, 2c.) 
IY.—MOULD PRODUCING A BROWN PIGMENT. 
This was found by us producing a dark-brown pigment on a gelatine-plate which 
had been exposed to the air on Norwich Cathedral. 
Microscopic appearance .—It forms a tangled network of branching filaments 
without visible division; in thickness it goes up to '8p. In Plate 18, fig. I, No. id, 
the filaments are shown magnified about 600 times. No spore-formation has been 
observed. 
Appearance in Cultivations. 
Gelatine.-— After a few days the needle-track below the surface exhibits small, 
isolated, fiocculent centres. The surface is depressed and exhibits greyish fiocculent 
