LEPIDOPTEROUS PUPH1 AND THEIR SURROUNDING SURFACES. 
349 
Dates, &c. 
(a) 3 larvae transferred to gilt box for all or 
part of Stage III. 
(/3) 3 larvae transferred to black cylinder for 
all or part of Stage 111. 
Aug. 26, 10.55 A.M. 
„ 4.30 p.m. 
„ 5.30 p.m. 
„ 7.50 p.m. 
„ 9.10 p.m. 
„ 11.30 p.m. 
Aug. 27, 9.40 a.m. . 
3 suspended larva; transferred from 
food-plant to gilt box 
Still suspended. 
1 pupating. (Thus 6% hours of Stage 
III. were passed in the box.) 
No change. 
Another pupated about 8.30 p.m. (Thus 
about 101 hours of Stage III. were 
passed in the box.) 
No change. 
Last pupated many hours, about 4.35 
A.M. (Thus about 17J hours of Stage 
III. were passed in the box.) 
3 larvte, suspended to a clear glass roof, 
transferred to black cylinder. 
Still suspended. 
11 11 
11 11 
11 11 
11 11 
All 3 pupated many hours, about 4.35 
a.m. (Thus about 17f hours were 
passed in the dark.) 
Aug. 29, ~) 
Results of com- j 
parison together ! 
and with all exa- [ 
mined on this | 
date. J 
All 3 pupae were (3), one of them a 
little darkish, and none quite so light 
as the (3) in the dark cylinder, but 
very little difference between any of 
the (3) in gilt box or dark cylinder. 
1 of the 3 pupae was a dark (2). 
1 „ „ (3). 
1 „ „ lightish (3), 
- rather more gold than usual. 
3 
Comparison of the dates shows that two of the larvae in the box cannot have passed 
the first and presumably the sensitive part of Stage III. under the influence of gilt 
surroundings, and it is by no means certain that the third larva pupated at a time 
near the hour which is selected, for the limits were very widely separated, and the 
estimate therefore becomes exceedingly rough. The three larvae in darkness all 
pupated in this wide interval of time, but here it becomes more probable that one or 
more of them passed almost the whole of Stage III. under the new conditions, and 
we find that one of them is very dark. It must be remembered that the latter larvae 
had been previously exposed to plenty of light, and were not among dark sur¬ 
roundings, whereas the former were much shaded and among the dark leaves of a 
large amount of food-plant. The experiment, as far as it goes, certainly favours the 
view that the larvae are sensitive during part of Stage III., although, standing alone, 
it would be totally insufficient as evidence. It is noteworthy that the larva in 
darkness was the only (2), except one, obtained in the whole of this series. 
■ Thus 52 pupae were obtained in this series. 
VII.—A small number of larvae (12), the remnant of a company, were found 
August 26 on a nettle-bed in Binsey churchyard, and four others, certainly 
belonging to the same company, were found on the church itself in the prepara 
tory period. The negative results obtained with the shorn larvae induced me to 
again attempt the blinding experiments with these larvae also, for the results of the 
last series had not been obtained. 
