LEPIDOPTEROUS PUPH1 AND THEIR SURROUNDING SURFACES. 
361 
C. Another small transference experiment was made with the three larvse found 
suspended to the food-plant at 2.30 P.M., August 31, when most of the larvae were 
re-arranged. These three were then transferred to the gilt box, two being pinned up 
against the side and one on the roof. 
September 1, 9.30 a.m. All had now pupated; the one on the roof last night, 
and the two on the side some hours. 
September 3, morning. Comparison produced these results :— 
Of the 3 pupae, 1 which pupated evening of Aug. 31 (on roof) was (1), very black pigment. 
,, 3 „ 2 ,, later (on side) .... 1 „ (4), less gold than usual. 
1 „ (5), normal gold. 
When these three suspended larvae were transferred August 31, one pupa was also 
found suspended from the food-plant: it was a light (3). 
These results harmonise well with the more complete transference experiments in 
Division B. The dark transferred pupa was only exposed in the larval state for the 
last few hours of Stage III., and the resulting colour shows that it could not have 
been influenced at all. It was evidently an exceptionally dark form for this series. 
The others were exposed for probably the whole of Stage III. and were much affected, 
one of them being brighter than any of the transferred pupae in Division B. The 
latter result must be also partially due to the fact that the larvae had been previously 
exposed to surroundings which were far less dark than those made use of in B. 
D. Another experiment was made upon six mature larvae, which were placed, 
August 31, 2.30 p.m, , in a small cylinder resembling those described in Series V., B., 
covered with two layers of black tissue-paper, but lined with gilt paper. The larvae 
had the opportunity of being influenced by the gold, illuminated by daylight, for 
a few seconds before the cylinder was placed on a black floor, with another double¬ 
layered black cylinder over it, in a dark cupboard. 
September 2, 7.30 p.m. The cylinder was not touched till this date, but I had 
estimated that they would have pupated long before this time, and therefore the 
cylinder was now removed, and it was found that all six had pupated on the roof and 
had taken their final colour. 
September 3, morning. The pupae were compared with all the others, giving these 
results ;— 
Of the 6 pupse, 3 were light (3). 
1 was . . (4), hut with little gold. 
1 ,, . . (5), not much gold for this degree. 
5 
The colour of the 6th pupa was accidentally omitted from the notes. 
It is possible that these results were influenced by the few seconds of exposure to 
the gilt in a bright light, or by the exceedingly small amount of light which may have 
penetrated. But, on the other hand, the whole series tends strongly towards the 
MDCCCLXXXVII.—B. 3 A 
