SWARMING AND TITVINO. 
127 
HO firmly to her body, that it could not be lemoved «dth. 
out tcariii" her to jiieccs. 
The following facts will show that the impregnation of 
the queen by the drone, in the open air, may be made a 
matter of ocular demonstration: Lewis Shrimplin, of 
Wellsboro’, Brook County, Virginia, purchased a mova¬ 
ble-comb hive, in the Spring of 185 V, into which he put a 
second swarm. Finding, after a few days, that the bees 
had built a number of very straight combs, he called some 
of his neighbors together, to witness the ease wth which 
he could take out, and replace their combs. While stand¬ 
ing in front of the hive, he saw the queen coming out, and 
the idea occurred to him to catch her, and tie a very fine 
silk thread to one of her thighs. This he accomplished 
successfully; and as she began to ascend,* the drones 
by which means these can act by pre»mr€^ in the interior of the body of the bee, 
upon the neipliboring penis which Is to be protruded.” 
“The following Interesting experiment” (Parthenogenesis, p. 54) was made by 
Borlepsch, in order to confirm the drone-productiveness of a virgin queen. He 
contrived the exclusion of queens at the end of September, 1854, and, tlicrefore, at 
a time when the.ro was no longer any males; ho was lucky enough to keep one of 
them through the Winter, and this produced drone-oflfspring on the 2d of March, in 
the following year, furnishing fifteen hundred cells with brood. That this drone¬ 
bearing.queen remained a virgin, was proved by the dissection which Louckart 
undertook, at the request of Berlepsch. lie found the state and contents of the 
seminal pouch of this queen to be exactly of the same nature ns those found In 
virgin queens. The seminal receptacle In such females never contains semen- 
masses, with their characteristic spermatozoids, but only a limpid fluid, destitute 
of cells and granules, which Is produced ft-om the two appendicular glands of the 
seminal cajisule; and, os I 8ui»pose, serves the purpose of keeping the semen 
transferred into the seminal capsule In a fresh state, and the spermatozoids active, 
snd, consequently, capable of impregnation.” 
By referring to pages 88, 89, the reader will see that Prof. Leldy dissected for 
mo a drone-laying queen, nearly three years before this examination <‘f I-euckart 
Prof. Slebold, in 1848, examined the spermathoca of the quoon-bee, and found It 
aftpv copulation, filled with the seminal fluid of the drone. At that time, Apl- 
•lians paid no attention to his views, but considered them, as ho says, to bo :)nly 
stufy It seems, then, that Prof. Lelily’s dissection (pp. 84, 85) was 
Hot, a.s I had hitherto supposed, the first, of an impregnated spcrmatheca. 
* Dzlorzon supposes that the HoutuJ i)f the qiu'en's wings, when she is in the 
air, o.xcUes the drones. In the interior of the hive, they are never scon to noUefl 
