18 
Ornithodorus savignyi 
tick was being studied at Pusa, but their results are apparently as yet un¬ 
published. Nuttall and Warburton (1908) only note that “there are at least 
two nymphal stages, if not more.” These facts and also that 0. savignyi is a 
potential disease carrier justify the publication of these incomplete notes, 
made under laboratory conditions. This investigation did not yield the same 
amount of information as was obtained in the case of 0 . moubata, as the two 
were run together, and it was not possible to rear successfully large numbers 
of individuals of both species. As would be expected, the results of this second 
investigation are, for the most part, in agreement with those of the first, and 
where confirmation is complete, discussion of the records is omitted for 
economy of space, the reader being referred to the paper on 0. moubata. 
SECTION I. BIOLOGY OF 0. SAVIGNYI. 
It is unnecessary to recapitulate the experimental procedure, which was 
the same throughout as that adopted for 0. moubata. Similar also was the 
aim of the investigation, namely, the determination of the number of nymphal 
stages passed through by this tick before reaching maturity, of the effect of 
temperature and moisture on the duration of the stages and of the changes 
taking place in the external anatomy of the ticks at each stage of develop¬ 
ment. As before, some notes were made on oviposition, copulation, longevity 
and engorgement. Females, with their progeny, from each stock were reared 
separately, but their records, being similar, are not shown separately in the 
following synopses of results. 
Experimental records relating to females kept at different temperatures. 
Three series of female ticks were maintained at 22°, 30° and 37° C. re¬ 
spectively, but only the second series bred at all successfully, as shown in 
Synopsis I. 
Females and progeny maintained at 22° C. 
A series of six females, which emerged from the last nymphal stage between 
May and July, 1913, although they fed well and were fecundated on one or 
more occasions, failed to oviposit until about the 400th day. The larval yield 
was too small to promise success in rearing and the experiment was discon¬ 
tinued. Females which emerged about the same time from the same stock, 
reproduced very successfully at 30° C. However, it is considered that a 
repetition of this experiment would show that 22° C. is not too low a tempera¬ 
ture for reproduction by this species. 
