The nuclei in the amoebulae could only be studied in stained preparations. 
Staining shows that the nuclei of the two sporoplasms remain independent 
in the amoebula after the fusion of the cytoplasm, although they are found 
lying side by side (Figs. 4, 7). 
Further observation on the amoebula could not be made either in hanging 
drop preparations owing to bacterial contamination, or in the animal body, 
although the sectioned stomach and intestine of four host animals which had 
been fed with the infected kidneys or cork, were carefully studied. In one 
amoebula shown in Fig. 9, which has been seen in a hanging drop preparation 
after 22 hours, the two nuclei were lying together, indicating that in the next 
stage a complete fusion of the two nuclei might occur. 
Apparently, stages nearest to this were observed in the space between the 
glomerulus and Bowman’s capsule of Malpighian bodies and in the uriniferous 
tubule below the Malpighian body of infected kidneys of R. pipiens. Quite 
frequently stages such as shown in Figs. 12-17 were observed in these parts of 
the kidney, although none were found to be in the epithelial cells of the tubule 
or in the surrounding connective tissue of the kidney. I consider the stage 
shown in Fig. 12 as that in which the nuclei of the amoebula are completing 
the fusion and those shown in Figs. 13-17 as those in which stages of schizogony 
of the young uninucleate amoebulae are represented. Since I have not seen 
the schizogony in the fresh state, I cannot state definitely that it occurs. 
Judging from the number of the parasites found, I am, however, inclined to 
think that schizogony also takes place beside the process of gemmation which 
occurs at a later stage of development. 
The young uninucleate form is rounded with deeply staining reticulated 
cytoplasm and a large nucleus. At first the nucleus seems to be rich in the 
nuclear sap and to be surrounded by a somewhat indistinct membrane. The 
chromatic substance is collected in one place in the form of a karyosome (Figs. 
17, 18). Owing to the variable shapes it assumes (Figs. 18, 19), the young 
parasites seem to undergo amoeboid movements. The cytoplasm is uniformly 
reticulated, often showing more or less large vacuoles (Fig. 19). As the body 
grows, the nucleus undergoes changes. The nuclear membrane becomes now 
distinctly visible and the karyosome more compact. The achromatic network. 
on which chromatin granules are scattered, becomes clearly observable (Figs. 
19, 20). 
The germination of the myxosporidian spores has undoubtedly been one of 
the important objects of study to investigators. I shall quote some records 
on the subject in species of similar habitat. Auerbach (1910) figured the 
separation of the shell-valves of a spore of Myxidium bergense which had been 
introduced into the intestine of one of its hosts, Gadus virens, and further 
stated that when spores were taken from the stomach and mixed with a drop 
of the host s bile on a slide, the polar filaments became extruded, and that 
“nach 20 Min. bis J Stunde kann man auch leere Schalen finden, ein Zeichen, 
dass die Amoeboidkeime ausgekrochen sind.” Davis (1916) observed that 
