R. Kudo 235 
In the spores of several species of Myxosporidia, which I have studied up 
to the present (Kudo, 1920a, 19216), I have always seen a single binucleate 
or occasionally uninucleate sporoplasm in each individual. 
On examining the spores of L. ohlmacheri, I was surprised to find that each 
spore contained always two sporoplasms. Even in a fresh condition, one can 
distinctly recognize two sporoplasms in every spore, young as well as mature, 
clearly separated from each other (Figs. 55, 87, 90, 94, 96, 97). They are 
rounded and distinctly contoured. They may be of unequal form in one spore, 
yet their volume appears to be approximately the same. In some spores, they 
may be small and remain as rounded masses in the posterior portion of the 
spore (Figs. 87, 97), while in others they may be comparatively large and 
occupy more than half the cavity, extending anteriorly around the polar 
capsules (Figs. 88, 90). In the fresh state, the sporoplasms are of uni¬ 
formly homogeneous hyaline cytoplasm destitute of the usual fine granulation 
found in the sporoplasm of spores of other species. They, however, contain 
small refractive globules of variable size and number (usually from three to 
five) which are of fatty nature. The nucleus can ordinarily not be observed 
in the unstained state. Another strange feature of the sporoplasms is their 
strong affinity for nuclear stains. W hen the spores in smears are fixed and 
stained by any of the stains used, the sporoplasms often withstand the de¬ 
coloration, and remain deeply stained, in which case the detection of their 
nuclei is impossible. 
As far as I am aware, Erdmann (1917) is the only investigator who, dealing 
with Chloromyxum leydigi, has concluded that “die junge einkernige Chloro- 
myxidie ist aus einer Anlage entstanden, die zwei Zellen und zwei Kerne in der 
Sporenhulle hatte.” She has figured three stained spores of the species, which, 
however, do not illustrate the statement distinctly. Aside from Erdmann’s 
observation Thelohan (1895) mentioned that in the spore of Ceratomyxa 
sphaerulosa the “protoplasm n’occupant qu’une des valves, dans l’autre 
valve, on voit d’ordinaire de petit amas dune substance tres pale(x).” 
Whether this substance represented the second sporoplasm or not remains 
unsettled. 
Although the size of two spores formed in one trophozoite is usually similar, 
spores from different individuals may vary considerably. Measurements of 
a large number of spores gave the following average dimensions: Fresh spores: 
sutural diameter and thickness 9-5-12/i, breadth 13-14-5/1, diameter of polar 
capsules 3-4-5/i, length of extruded polar filaments 42-62/1. Stained spores: 
sutural diameter and thickness 8-10/z, breadth 9-12/x, diameter of polar 
capsules 3-4/x. 
These dimensions differ greatly from those of Ohlmacher’s form, since 
this author records the sutural diameter and breadth of spores in sectioned 
preparations as 6/x and 8/x respectively. It is, however, understood that the 
section preparations always give much smaller dimensions of spores or tro¬ 
phozoites compared with those of stained smears or particularly of fresh 
