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Biology of Trypanosoma equiperdum 
The experimental mice (four) inoculated subcutaneously with the contents 
of the vesicula seminalis of mice harbouring the trypanosome in their blood 
(diluted with physiological salt solution) remained healthy after being under 
observation for more than a month. On the other hand, the control mice 
inoculated subcutaneously with saline and the blood of the corresponding 
mice infected with T. equiperdum, showed trypanosomes in their blood after 
6-7 days, and succumbed on the 9th-10th day. 
Experiments conducted on a dog infected with T. equiperdum showed that 
this trypanosome does not pass into the sperm even at the period of the 
highest development of the disease. 
Mice inoculated subcutaneously with the sperm of the infected dog at 
different periods of the disease remained healthy (under observation for more 
than 40 days), whereas the control mice inoculated subcutaneously with saline 
and blood from the same dog showed trypanosomes in their blood on the 
4th day and died on the 7th-8th day. These experiments are illustrated in 
Table I. 
Table I. 
Mice inoculated subcutaneously with the sperm of a dog infected with 
T. equiperdum. The presence of trypanosomes in the blood was 
established by microscopical examination. 
Result of examination of blood of Results ol 
Infected animal Experi- inoculated animals on days further bloc 
and material used mental r --—•- A -—examinations 
for inoculation animal 1 2 3 4 5 6 7 8 9 10 11 12 13 14 inoculated m 
1. vi. 1917 
Dog’s (“Gutik”) Mouse - -- -- -- -- -- -- - Blood exam 
sperm ,, - during 40 d 
Blood of the same ,, — — — + + + (died) No trypanosc 
dog (control exp.) found 
5. vi. 1917 
Dog’s (“Gutik”) Mouse - -- -- -- -- -- -- - Blood exam 
sperm ,, - - - - for more the 
»> -------------- month with 
>> - suits as abov 
Blood of the same ,, --- + + + + (died) 
dog (control exp.) 
10. vi. 1917 
Precipitate of cen- Mouse - Blood exam 
trifuged dog’s „ - three weeks 
(“Gutik”) sperm ,, - the same rest 
99 — — — — 
Blood of the same „ --- + + + (died) 
dog (control exp.) 
It was very difficult to obtain the sperm for inoculation into mice on 
June 10th from the dog “ Gutik, ” as the animal was already very weak. Seven 
days later the dog died. | 
The experiments conducted with the view of discovering if T. equiperdum 
could traverse the intact mucous membrane, were carried out on eleven mice ,i 
and five rabbits, as follows: A mixture of 1 c.c. of physiological salt solution 
and five drops of blood (from the tail of a mouse infected with T. equiperdum) 
