Gr. H. F. Nuttall 
19 
(b) Operations on the nymph and their effect on the adult. 
Legs were amputated in three nymphs (Nos. 8-10) on 3. viii. 15 as follows: 
In No. 8 legs III and IV had 3 and 4 articles removed respectively; in 
No. 9 legs II and III lost 3 and 4 articles respectively; in No. 10 leg I had 
4 articles cut off. These ticks in due course moulted and gave rise to three 
females. 
AH of the previously amputated limbs were regenerated in the adults, 
only in No. 9 were legs II and III (those mutilated) slightly smaller than 
normal. 
3. Hyalomma aegyptium. 
Operations on the nymph and their effect on the adult. 
The legs were amputated in seven nymphs (Nos. 11-17) as follows: 
Hyalom¬ 
Number of articles 
ma No. 
amputated from legs 
Regeneration in adult 
11 
2 from leg III 
perfect 
12 
2 from leg IV 
99 
13 
4 from legs II and III 
„ (untouched opposite leg III small) 
14 
4 from leg I 
99 
15 
3 from leg II 
regenerated, distal articles very slightly shorter than 
normal 
16 
4 from legs I and II 
regenerated, slightly shorter than normal 
17 
3-4 from legs I, II, III, IV regenerated, legs II, III, IV somewhat smaller than 
normal 
SURVEY OF THE RESULTS OBTAINED. 
All the immature ticks in these experiments were operated upon within 
1-2 hours of their becoming fully gorged and abandoning the host. Extensive 
mutilations may cause death through excessive loss of coelomic fluid. Moderate 
mutilations are well borne by ticks, 78 out of 108 survived the operations 
herein described; the heaviest loss followed operations affecting the basis 
capituli. One third-stage nymph (Argas 73), in which the basis had been cut 
across midway in the second nymphal stage, survived for four years in the 
laboratory although it was unable to feed because of its mutilated mouth- 
parts 1 . Metamorphosis may be retarded or not according to the severity of 
the mutilation that has been inflicted. 
REGENERATION OF MOUTHPARTS. 
Argas persicus. 
Operations on larvae wherein the mouthparts are mutilated shortly 
after the larva has abandoned the host in a fully gorged condition, cause 
the various structures to be differently affected in the first-stage nymph: 
the palps are not regenerated but appear as stumps which but for their closed 
and rounded ends correspond mostly in structure to the parts that were left 
intact in the larva (Fig. 4 A-E); occasionally an additional article or two is 
1 See p. 24, 
