18 
Regeneration in Ticks 
In my experiments the legs were amputated in immature stages of 
Amblyomma hebraeum, Hyalomma aegyptium, and in a few Argas persicus for 
purposes of comparison: 
1. Argas persicus. 
(a) Operations on the larva and their effect on the first-stage nymph. 
Legs were amputated in three larvae (Nos. 23, 24, 26) on 23. ii. 15. In 
No. 23 legs I—III on the right side had 4, 3J, and 4 distal articles cut off 
respectively; in No. 24 leg III had 21 articles removed; in No. 26 leg 1 had 
4 articles removed. 
In none of these larvae were the limbs regenerated in the first-stage 
nymph, all of the limbs ended in stumps corresponding to the seat of amputa¬ 
tion in the larva. No. 26 moulted as a first-stage nymph on 17. iii., it fed on 
25. iii., and on 19. iv. it was found to have moulted with all its legs normally 
formed. 
(b) Operations on the first-stage nymph and their effect on the second- 
stage nymph. 
The legs of the right side were amputated in three first-stage nymphs 
(Nos. 45-47) on 25. iii. 15. In No. 45 legs I and IV had 5 articles removed; 
in No. 46 legs I and II had 5 and 3 articles removed respectively; in 
No. 47 legs I and III had 4 articles removed. The three ticks moulted to 
second-stage nymphs on 30. iii.—2. iv. and fed normally on 28-30. iv. 
In the second nymphal stage, No. 45 had legs I and IA of subnormal size, 
and No. 46 had legs I and II smaller than the corresponding legs on the side 
that had not been mutilated. Through an oversight No. 47 was allowed to 
moult and the moulted legs were lost so that its condition escaped obser¬ 
vation. 
After further feeding, Nos. 45 and 47 moulted to third-stage nymphs 
whilst No. 46 emerged as a normal skipping the third nymphal stage 
which is more commonly omitted in normal ticks of this species. No. 47 gave 
rise to a $. In all of these stages the ticks appeared perfectly formed, no 
difference in size between corresponding legs being observable. 
2. Amblyomma hebraeum. 
(a) Operations on the larva and their effect on the nymph. 
Legs were amputated in three larvae (Nos. 11-13) on 27. vii. 15 as follows: 
In No. 11 the greater part of legs II and III was removed; in No. 12 leg I 
had 5 articles removed; in No. 13 leg III had 5 articles removed. 
Examined after they had moulted, the nymphs were found to have re¬ 
generated their legs perfectly, the previously mutilated limbs not being 
smaller than normal. 
