Gr. H. F. Nuttall 
17 
3. Hyalomma aegyptium. 
Operations on the nymph and their effects on the adult. 
Seventeen nymphs were operated upon on ]. vi. 1915, after feeding and 
dropping from a hedgehog. -They all moulted between 17 and 22. vi., except 
No. 17 which moulted on 24. vi. 15. 
Nature of operation on nymph 
Palp 
■"x 
Hyalom- articles 
Ilypostome 
4 
Clielicerae 
ma N o. removed 
cut 
Regeneration observed in adult £ or ? 
I 
R and L — 
nr base 
R and L cut 2 l 
$ right internal article of one digit slightly 
deformed, otherwise perfect 
2 
R and L — 
nr base 
R cut 1 l 
$ perfect 
3 
R and L — 
nr base 
R cut 2 l 
$ perfect but for deformity of external 
L cut 1 l 
article of right digit 
4 
R and L — 
nr base 
R and L cut 2 l 
$ perfect 
5 
R and L — 
at base 
R cut 3 l 
$ perfect 
6 
R cut 2-4 
L 
nr base 
R and L cut 2 l 
<3 perfect 
7 
R cut 1-4 
nr base 
R cut 
$ perfect but for deformed R internal article 
L — 
of digit 
8 
R and L cut 2-4 
at base 
R and L cut 4 l 
$ R and L digits badly deformed, rest 
normal 
9 
R and L — 
nr base 
R cut 
$ perfect 
10 
R and L — 
nr base 
R and L cut 1 l 
$ perfect 
II. REGENERATION AFTER AMPUTATION OF THE LEGS. 
(Experimental Records.) 
The experiments of Hindle and Cunliffe (cited on p. 7) on the mutilation 
of the legs in Argas persicus may be summarized as follows: 
A\ hen the legs of a freshly gorged larva are amputated, they are not 
regenerated or are imperfect in the first-stage nymph. If the larva is operated 
upon whilst on the host, that is 2-3 days before it would drop off gorged if 
left unmolested, the legs may at times be regenerated after the tick abandons 
the host. This difference in the behaviour of the larva under the two condi¬ 
tions specified is attributed to nymphal development proceeding within the 
larva whilst it is upon the host and developing nymphal tissues being injured 
when gorged larvae are operated upon. First-stage nymphs that were muti¬ 
lated in the larval stage, when raised, without further operative interference 
to second-stage nymphs, were found to have regenerated the amputated 
limbs although these were usually of subnormal size whilst perfectly formed; 
after a further moult, the ticks became normal. Experiments were also made 
with nymphs. It was found that leg regeneration occurred in all immature 
stages where amputation took place sufficiently long before moulting, but 
the legs were usually of subnormal size. 
Parasitology xii ■> 
