G. H. F. Nutt all 
9 
These permanent preparations, ten or more per slide, served as accurate 
checks to graphic notes that were made of each operation. The skins of the 
ticks as they moulted after operation, or the ticks themselves, were mounted 
serially in a similar manner, air being excluded from the mounts by taking 
the usual precautions. The diamond circles greatly facilitate the finding of 
minute objects mounted within their contours 1 . 
The ticks, after operation, were confined separately in numbered tubes 
standing in racks within a thermostat at 30° C., whence they were taken 
daily for inspection; the cast skins were periodically removed and mounted in 
the manner previously described. 
The abbreviations used in the tables that follow are 
R and L denoting Right and Left in respect to the parts that were amputated or left 
untouched. 
— (a dash) denotes that no operation took place on the parts indicated. 
The degree of amputation undergone by the several parts is denoted in 
different ways: 
(a) by fractions of the length of the part (hypostome, palps) from apex to base, i.e. 
1/2, 1/3, etc. 
( b ) by stating the number of articles that were removed wholly or partly, reckoning 
the basal article as 1 (palps have 4 articles 1-4) or by recording the number of distal 
articles removed (legs have 6 articles including the coxa). 
(c) Operations where digits were removed without appreciable injury to the shaft 
of the chelicerae, are indicated by cut d ; where the shaft was cut across, the amount 
thereof removed is given in lengths of digit taken as a measure, thus cut 4 l denotes that 
the portion of shaft removed with the digit was four times the length of the digit. 
1 I have devised another simple method of mounting large numbers of small objects in series. 
The method consists in mounting each small object in a numbered cell in balsam. To do this, 
cardboard of suitable thickness is cut in oblongs that are shorter (to leave room for a label) and 
nearly as wide as a slide. Two rows of circular holes (ca. 6 mm.) totalling 10-12 in number, are 
neatly cut in the card with a sharp hand-punch and serial numbers are written in Indian ink on the 
card beside the holes. Before use, the holed cards are immersed in xylol for 24 hours and afterwards 
in balsam for 24 hours; they are then transferred to a slide, the objects are placed in the holes 
and these are filled with balsam. A coverglass, of a size to match the card, having been dipped in 
xylol, is at once placed in position, the use of xylol helping to exclude air bubbles. The method is 
very convenient, economical, and readily lends itself to modifications. 
