131 
C. H. Treadgold 
and covered with a fine powder which, on examination with the oil immersion, 
resolved itself into large numbers of small bacteria measuring about 3 by 1 
these were, tor the most part, arranged end to end in short rows and covered a 
large portion of the surface of the body. The general body-cavitv was packed 
in many places with granular debris, while the mid-gut was in a state of atony, 
its posterior half measuring in places more than 300p, in diameter instead of 
the normal 120 to 180/z. 
The other two were also slightly infected in the same way. An attempt was 
made to cultivate these organisms in broth and on agar slopes, but the media 
remained sterile. 
The absorption and calcification of dead Loas by the host. The calcification 
of dead Loas has been occasionally reported in autopsies on the human subject. 
In Papio cynocephalus, from 5 to 10 per cent, of the Loas found at autopsy 
were dead and calcified: the greatest number ever found in one baboon was 
forty-three, of which three had undergone this process. Whether they can be 
absorbed without undergoing calcification, I am unable to say. 
D. The Influence of these Parasites on their 
Respective Hosts. 
Traumatic action. In the living worms of both sexes, the mid-gut often 
showed through the cuticle as a bright-red streak owing to recent blood ab¬ 
sorption; this does not seem to have been noted for L. loa. 
Toxic action. Although no localised oedemas comparable to “Calabar 
swellings'’ were ever noticed, I am inclined to think that, in moderately or 
heavily infected animals, the subcutaneous fat was diminished in quantity 
and paler than normal. An investigation of the possible toxicity of L. papionis 
for other animals seemed therefore desirable, and the following experiments 
were made on guineapigs. 
Experiments. Three healthy adult male guineapigs, whose blood had been 
examined on many occasions during the preceding three months, were chosen. 
Two male and two female Loas were introduced under the skin of the back 
of guineapig no. I, but the stitches not being sufficiently close together, two 
of them escaped shortly afterwards. Two males and three females were in¬ 
sinuated under the skin of the back of guineapig no. II, care being taken to 
remedy the defect in technique. Guineapig no. Ill served as a control. A week 
later, no. II was found to be greatly emaciated, while the other two remained 
fat and healthy. No. II had always showed a slight lymphocytosis, and this 
had now become accentuated; but the percentage of eosinophiles was not 
increased. The animal died on the ninth day, having lost sixty grammes in 
weight during the last 24 hours. At the autopsy, which took place about 
12 hours after death, four Loas were found out of the five which had been 
originally introduced. One female was discovered in the left lumbar region 
between the peritoneum and the muscles of the back, a second beneath the 
