378 
ENDOLIMAX KUENENI N.SP., PARASITIC IN THE 
INTESTINAL TRACT OF THE MONKEY 
MAC AC US CYNOMOLGUS. 
By S. L. BBUG, 
Major , Dutch-lndian Army Medical Service. 
(Centraal Militair Geneeskundig Laboralorium, Weltevreden, Java.) 
(With Plate XXIII.) 
This amoeba was found in the large intestine of a Macacus cynomolgus, that 
was killed because it suffered from paralysis of the hind legs and emaciation. 
The autopsy showed these symptoms to be dependent on a general tuber¬ 
culosis, in which, besides many other organs, the spinal matter was involved. 
Microscopic examination of the intestinal contents revealed the presence of 
motile amoebae and cysts. Except some small tubercular foci in the serosa 
no pathological lesions could be detected in the intestine. Close examination 
of the mucosa failed to reveal any ulceration; blood and mucus were totally 
absent. 
When alive, the motile amoebae did not show a distinct nucleus. The 
ectoplasm was only visible where pseudopodia were being formed and it was 
apparently absent in the resting amoeba. The endoplasm was vacuolated, 
some vacuoles containing small food-particles, mostly bacteria. The amoebae 
measured 7-12/z when rounded. The living cysts closely resembled iodine- 
cysts as described by Wenyon and O’Connor (1917). When the cysts were 
treated with Weigert’s iodine solution, the resemblance with iodine-cysts was 
emphasised by the appearance of a dark reddish brown stained vacuole 
measuring of the cyst’s diameter. Moreover, just as in iodine-cysts, 
Weigert’s solution failed to produce a clear nuclear picture. The diameter of 
the cysts varied between 7 and 10 p,; they were round or oval-shaped. 
On staining with Delafield’s haematoxyline or according to Heidenhain’s 
method as modified by Brug (1919), the amoebae showed the nuclear struc¬ 
ture which Kuenen and Swellengrebel (1917) consider to be characteristic for 
the genus “ Endolimax ” (Plate XXIII, Figs. 1-3). The resemblance of the cysts 
still held good in stained films. The great majority of the cysts from the monkey 
were mononuclear, binucleate cysts were very rare (Fig. 8). The nucleus con¬ 
sisted of a darkly staining, large, homogeneous looking, excentrically situated, 
round caryosome, on one side surrounded by a crescent-shaped, less intensely 
coloured mass. The latter showed a granular structure more distinctly in 
