S. L. Brug 
Delafield preparations (Fig. 4) than in Heidenhain-stained films (Fig. 5). 
Caryosome and crescent were separated by a narrow clearly stained area. 
The iodophil vacuole appeared as an empty area. The cysts had a double 
contour, the outer limit of the cyst-wall being visible as a faintly coloured 
line. 
The stained cyst almost always showed a structure which is absent in 
human iodine cysts. In the protoplasm there occurred a sharply limited area, 
round or somewhat irregularly shaped, whose periphery stained more darkly 
than the surrounding protoplasm whilst the centre stained like the proto¬ 
plasm (Plate XXIII, Figs. 4, 6). In the minority of the cysts this structure 
stained homogeneously, without a clear centre (Fig. 7). 
But for this darkly staining protoplasmic area in the cysts from the 
monkey’s intestine these might be considered identical with the iodine-cysts 
of human origin. The constant presence of the darkly staining area in the 
monkey’s parasite and its absence in the human parasite, suffices to differen¬ 
tiate these two amoebae. I therefore regard the former as a separate species, 
for which I propose the name Endolimax kueneni. 
REFERENCES. 
Brug (1919). La coloration des entamibes intestinales des selles. Bull. Soc. Pathol. Exot. 
xn. 71. 
Kuenen and Swellengrebel (1917). Korte beschrijving van enkele minder bekende 
protozoen uit den menschelijken darm. Gen. Tijdschr. voor Ned.-Indie, lvti. 496. 
Wenyon and O’Connor (1917). The character and diagnosis of the various intestinal 
protozoa in man in Egypt, etc. Journ. Roy. Army Med. Corps, xxvm. 152. 
Parasitology xii 
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