32 
beginning from such accidental finds, by exposing healthy insects 
(cater pillars by preference) to contagion from the dead insects 
and making the artificial cultures afterwards from these purposely 
infected specimens. 
The following table will serve as a synopsis of the data upon 
which the foregoing statements concerning sterilized cultures* are 
based. The figures in the first column refer to the detailed de¬ 
scription of experiments following, and those in columns four to 
eight represent the number of days after infection: 
Table of Sterilized Cultures of Sporotrichum globuliferum, 1891 . 
No. 
Date. 
Medium. 
Visible 
growth. 
Surface 
covered 
Spore- 
heads 
formed. 
Yellow 
ibh. 
Itipe. 
Remarks. 
15 
June 
11 
Corn meal 
5 
8 
11-13 
32 
4 4 
11 
2 
— 
5 
7 
11-13 
68 
l l 
12 
2 
— 
5 
ii 
12 
87 
4 ( 
12 
2 
5 
8 
10 
15 
Blue mold. 
24 
4 4 
23 
0 
0 
0 
0 
0 
tartaric acid. 
25 
4 4 
23 
0 
0 
0 
0 
0 
5£ “ “ . 
26 
( 4 
23 
0 
0 
0 
0 
0 
5 % “ “ . 
27 
4 l 
23 
0 
0 
0 
0 
0 
3£ “ “ . 
91 
4 l 
30 
0 
0 
0 
0 
0 
Blue mold. 
92 
4 4 
30 
0 
0 
0 
0 
0 
4 4 4 4 
93 
July 
4 4 
3 
0 
0 
0 
0 
0 
Foreign fungi. 
78 
7 
2 
3 
— 
12 
14 
45 
4 4 
7 
2 
3 
10 
12 
— 
35 
4 4 
9 
0 
0 
0 
0 
0 
90-102° Fah. 
47 
4 4 
9 
1 
— 
— 
11 
15 
50 
4 4 
9 
1 
— 
— 
11 
15 
31 
4 4 
10 
2 
— 
— 
10 
14 
72° Fah. 
94 
4 4 
16 
2 
4 
8 
— 
— 
56 
Dec. 
21 
1 
— 
9 
14 
21 
61/ 2 months old. 
84 
4 4 
21 
0 
0 
0 
0 
0 
« .4 44 
95 
4 4 
21 
2 
7 
9 
14 
21 
5 “ “ . 
101 
4 4 
22 
2 
6 
— 
— 
— 
Foreign fungi. 
41 
June 
11 
Middlings 
2 
7 
5 
9 
11 
52 
4 4 
11 
2 
— 
— 
6 
11 
Very scanty. 
72 
4 4 
12 
4 4 
2 
— 
— 
6 
12 
• 
89 
4 4 
12 
4 4 
0 
0 
0 
0 
0 
Foreign fungi.. 
53 
4 4 
11 
Bran 
2 
5 
5 
6 
11 
54 
44 
11 
4 4 
2 
— 
5 
8 
9-11 
77 
4 4 
12 
4 4 
2 
— 
6 
n 
i 
13 
88 
4 4 
12 
“ 
0 
0 
0 
0 
0 
Profuse mold. 
39 
4 4 
11 
Sawdust 
2 
— 
— 
9 
— 
Very scanty. 
4 4 4 4 
40 
4 4 
11 
4 4 
2 
— 
— 
9 
— 
71 
4 4 
12 
4 4 
3 
0 
8 
10 
— 
4 4 4 4 
90 
4 4 
12 
4 4 
0 
0 
0 
0 
0 
Profuse mold. 
1-3 
3-7 
5-10 
7-14 
11-21 
Unsterilized Cultures .—In the hope of avoiding the inconvenience 
and uncertainty of the sterilized-culture method, attempts were 
made to grow the Sporotrichum in the open air on suitable media, 
chiefly upon corn-meal mixtures, but also upon other substances 
used by botanists for the culture of fungi. These experiments 
failing,—evidently because of bacterial invasion of the material and 
consequent fermentation and decay,— additional trials were made 
with the same media variously acidulated with tartaric acid, in the 
hope that this treatment would so retard bacterial development as 
to permit a start of the Sporotrichum. As these experiments were 
all absolute failures, detailed discussion of them is unnecessary. 
* Agar cultures 7-10,13, and 14 are omitted from this table, because of a lack of detailed notes 
of growth. 
t 
