33 
Contagion and Infection Experiments* 
llith Chinch-hugs.— Our laboratory experiments for the con¬ 
veyance to chinch-bugs of the disease known as white muscardine 
number twenty for this year. Three of these, however, 57, 98, and 
100, are to be rejected on account of an infection of the check. 
Chinch-bugs died with muscardine in the lots separated in the 
beginning as a check on the experiment, thus discrediting the 
lesults in the expeiimental lot. Two others, Nos. 30 and 46, may 
also best be omitted, since, although bugs died in the experimental 
lot, the conditions and management were such that no assurance 
can be had that they died of muscardine. 
Arranging the remaining eleven in the order of promptness of 
the appearance of the Sporotrichum among the chinch-bugs, they 
stand as follows: ’ J 
No. 4. A contagion experiment; spores derived from dead 
chinch-bugs. First iusect dead with Sporotrichum in two days. 
No. 29. An infection experiment; spores from an artificial cul¬ 
ture four removes from dead insect. First appearance of Sporo¬ 
trichum, three days. 
No. 2. A contagion experiment; spores derived from dead beetles 
( Disonycha). First insect dead in three days; first appearance of 
Sporotrichum, eight days. 
No. 6. Contagion experiment; spores derived from dead chinch- 
bugs. First bug dead with Sporotrichum in five days. In this experi¬ 
ment the dead chinch-bugs from which the infection was derived 
were enclosed with the live chinch-bugs only twenty-four hours 
and were then removed. 
No. 3 Contagion experiment; spores derived from dead chinch - 
bugs. Infection temporary. Dead bugs removed in twenty-four 
hours. First specimens dead in five days; first development of 
Sporotrichum in nine days. 
No. 70. Infection experiment; spores from culture two removes 
(from cead insect. First bug dead with Sporotrichum in seven 
days. 
No. 99 Contagion experiment; spores from dead chincli-bugs 
recei\ed from Kansas. First specimens dead in ssven days' first 
appearance of Sporotrichum in thirteen days. 
No. 85. Contagion experiment; spores derived from dead chinch- 
bugs. Temporary infection; exposed forty-eight hours. First bug 
dead with Sporotrichum in nine days. 
No. 59. Infection experiment; spores fiom culture two removes 
lorn dead insect. First chinch-bug dead with Sporotrichum in 
eleven days. 
No. 97 Contagion experiment; spores derived from chinch-bugs 
received from Kansas State University. First bug dead in this lot 
in thirteen days; first appearance of Sporotrichum in twenty-four 
clays. 
reserved 
—3 8. E. 
