36 
From the foregoing it would again appear that there is little 
discernible difference in efficiency between spores of cultures de¬ 
rived immediately from the insect and spores of at least the fifth 
remove from the generation growing on an insect host. It is also 
evident that a variety of other insects, the soft-bodied larvae 
especially, are at least as susceptible to this fungous disease as are 
chinch-bugs, in connection with which it has been most frequently 
mentioned. A fuller discussion may well be postponed until the 
experiments of subsequent years are brought into comparison with 
those of 1891. Additional particulars may be obtained by the in¬ 
terested student from the outline of experiments following—the 
purpose of which is to show the order of dependence of succes¬ 
sive experiments—and from the detailed description of experiments 
containing all the essential particulars of the work for 1891. 
OUTLINE OF EXPERIMENTS WITH CHINCH-RUG MUSCARDINE FUNGUS 
( SPOROTRICHUM GLOBULIFERUM, SPEG.), NUMBERS 1-101, 
APRIL ‘27 TO DECEMBER 22, 1891. 
No. 1, April 27. Isolation experiment with chincli-bugs. 
No. 2, May 11. Contagion experiment with chinch-bugs and 
Disonycha. 
Nos. 3, 4, and 85, May 29 and June 1. Contagion experiments 
with chinch-bugs. 
No. 5, May 11. Contagion experiment with June beetles and 
Disonycha. 
No. 6, Mav 29. Contagion experiment with chinch-bugs, material 
from Dr. Snow. 
No. 7, May' 15. Agar culture of Sporotrichum from Prof. Thaxter. 
Nos. 8-10, May 18 to 20. Agar cultures. 
Nos. 11 and 12, June 20. Infection experiment with cliiucli- 
bugs and No. 10. 
No. 13, May 20. Agar culture. 
No. 14, June 4. Agar culture. 
No. 15, June 9. Corn-meal culture. 
Nos. 16-23, June 23 and 24. Open-air cultures on corn- 
meal mixtures. 
Nos. 24-27, June 24. Acidulated corn-meal cultures. 
No. 28, June 26. Open-air cultures on bread. 
No. 29, June 29. Infection of dead cliiucli-bugs. 
No. 30, July 6. Chinch-bug infection from Nos. 15, 32, 
and 54. 
No. 31, July 10. Corn-meal culture. 
No. 32, June 9. Corn-meal culture. 
No. 33, June 29. Cabbage-worm infection. 
No. 34, July 6. Chinch-bug contagion. 
No. 35, July 9. Corn-meal culture. 
