41 
under a bell-jar with green wheat and with several of the above 
beetles covered with fungus. May 14 first bug was dead; May 16 
another; and May 19 two more had died, covered with Sporotri- 
chum May 20, at 8 o’clock a. m., another chinch-bug was re¬ 
moved in the same condition. This insect had certainly been alive 
at 2:16 p. m., of the preceding day. May 22, two more fungus- 
covered-bugs removed, and May 23 an additional one. The prop¬ 
agation of the disease was rapid from this time to the k z9th, 
when all the chinch-bugs of the lot but one were dead and covered 
with Sporotrichum. Check lot without loss. 
No. 3. May 29. Contagion experiment with fresh chinch-bugs 
exposed to specimens dead with muscardine from No. 2. Thirty 
chinch-bugs collected at Litchfield, Illinois, were placed in a com¬ 
mon breeding-cage with wheat plants and two dead bugs from No. 
2 which were well covered with Sporotrichum. Twenty-four hours 
afterwards these two dead bugs were removed. June 3 one chinch- 
bug was dead; on the 7th two more had died and presented a 
visible growth of the fungus; Juue 10 two more of this lot were 
taken from the breeding-cage with muscardine fungus well devel¬ 
oped upon their surfaces. A check lot of an equal number sim¬ 
ilarly placed had not suffered at all. The bugs remaining in both 
these lots were now used in another experiment. 
No. 4. J une 1. A duplicate of No. 3, except that four dead 
bugs were placed with the living ones and were not subsequently 
removed. On the 3d of June one bug was dead and covered with 
fungus; on the 4th one more; and on the 7th two. The insects 
remaining were used for another purpose Juue 10. 
No. 5. May li. An infection experiment performed on June 
beetles (Lachnosterna) with spores from dead specimens of 
Disonychci pennsylvanica—t he same beetles used in No. 2. Several 
dead and two live June beetles were dusted with the spores and 
placed upon damp earth in a flower pot, which was then covered 
with glass. May 29 no growth of muscardine fungus; beetles 
dead and badly decayed, eaten in part by maggots of the genus 
Phora—as determiued by breeding. 
No. 6. May 29. A contagion experiment with chinch-bugs ex¬ 
posed to specimens dead with the white fungus received May 12 
from Dr. Snow of the University of Kansas. Thirty live chinch- 
bugs collected at Litchfield, Illinois, were put in a breeding-cage 
and supplied with wheat plants for food. Eight dead bugs from 
Dr. Snow were crushed and put in this cage, where they w ? ere 
left tw T enty-four hours. 
June 3 one bug was dead and covered with fungus; on the 7th 
three more had died, two with and one without the fungous 
growth; and tivo others dead with the disease were removed the 
10th. 
A check lot of an equal number was similarly placed. June 3 
one bug was dead in this check; and on the 5tli one more had 
