42 
died, but neither had any visible fungus on their bodies. June 
10 all the others in both lots were alive, and the insects remain¬ 
ing were used for other purposes. 
No. 7. May 15. A tube of agar containing a profuse growth of 
Sporotrichum globuliferum, fruiting abundantly, received from 
Dr. Roland Thaxter, of New Haven, Connecticut. The spores 
used in this culture were derived originally from caterpillars of a 
noctuid moth (Copipanolis vernahs), which Dr. Thaxter was 
rearing in a breeding cage. The culture was begun early in April, 
and was fully matured when received. 
Nos. 8, 9, and 10. May 18-20. Successive culture experi¬ 
ments, in test-tubes, on agar inoculated with spores from No. 7, 
all of which produced an abundant growth of Sporotrichum. 
These cultures were made as an accommodation in the botanical 
laboratory of the University of Illinois, and detailed notes were 
not made. 
Nos. 11 and 12. June 20. Infection experiments on young 
chinch-bugs treated with spores from No. 10. In the first 
(No. 11) several dozen young chinch-bugs, collected at Carlyle 
and Marissa, were placed in water, the surface of which had been 
previously dusted with spoies of the white fungus from No. 10. 
They were left for a few minutes, then removed and placed on 
growing corn under a gauze-covered glass cylinder breeding-cage. * 
In the second (No. 12) an equal number of young bugs from the 
same source w r ere placed in a dry bottle into which spores from 
No. 10 had been introduced, and left for a short time, after which 
they were enclosed in a breeding-cage similar to that used in No. 
11. June 22, no fungus in either cage; bugs in good condition. 
June 27, no growth. June 30, no fungus development. Experi¬ 
ments discontinued. Check lot of an equal number of bugs kept 
under similar conditions also remained without loss. 
No 13. May 20. Culture experiment precisely similar to Nos. 
8, 9, and 10, and made in the University botanical laboratory. 
A tube of agar treated with spores from No. 7 produced a pio- 
fuse growth of the white fungus. 
No. 14. June 4. A duplicate of No. 13, except that the spores 
used for inoculating the agar medium came from No. 13. Simi¬ 
lar results. Good fungous growth reported, but without details. 
No. 15. June 9. Test-tube culture experiment, the nutrient 
material being beef broth prepared as for the culture of bacteria, 
and used to saturate freshly heated corn meal to form a 
stiff batter. June 11, 8 a. m., medium sterilized for half an 
hour at 100° to 108° Cent, and treat* d with spores of muscardiue from 
No. 14. Kept at ordinary temperature of the room. June 13, 8 
a. m., fungous growth commenced; still more prominent on the 
14th and 15th; spreading freely on the lfith, aud filling all the 
small crevices on the surface of the medium; spores beginning 
* This breeding-cage was a cylinder of glass set in earth at the bottom and co\ered with 
gauze at the to w . allowing iree veutilutiou and evaporation. 
