43 
to form. Jane 17, the mycelial growth covering the entire sur¬ 
face. Heads of spores give it a distinctly granular appearance; 
medium drying out; 18th the same; 19th fungus slightly tinged 
with yellow—a sign of ripening of the spores June *20, medium 
dry enough to separate from tube, and fungus growing on under 
side; spores formiug in such places. June 22, spores can be 
detached by vigorous shaking; June 24, easily detached and ripe; 
surface brownish. 
The eight following (Nos. 16-23) are successive attempts made 
at the artificial culture of Sporotrichum in the open air upon 'ari- 
ous media, without resort to the sterilization methods of bacterial 
culture as given above. All were unsuccessful. 
Nos. 16 and 17. June 23. Culture exper ments, the nutrient 
material being corn meal soaked with an extract of the excrement 
of horses acidulated with tartaric acid, and inoculated wfith fungus 
spores from No. 15. Two quarts of fresh horse excrement stirred 
in two quarts of soft water, divided into two equal parts and 
cooked in separate water baths four hours; filtered twice tlnough 
cheese cloth; one (No. 16) acidulated wfith one half of one per cent, 
of tartaric acid, the other (No. 17) with a quarter of one per 
cent., and mixed with corn meal. Placed in glass dishes, treated 
with spores of Sporotrichum from No. 15, and covered with glass. 
June 25, covered with molds. June 2/, game condition; re¬ 
sults negative. 
Nos. 18 and 19. June 23 Culture experiments on solid horse 
excrement, boiled and filtered as in experiments 16 and 17, placed 
in tin pan3, inoculated with fungus spores from No. 15, and cov¬ 
ered with comm >n window-glass. The two experiments were pre¬ 
pared alike, except that No. 19 was slightly acidulated, while No. 18 
contained no acid. Juue 26 both were covered with mold. No 
Sporotrichum seen. June 27, same as yesterday. July 6, mold 
abundant. July 11, full of other fungi; no white fungus. 
Nos. 20 and 2L. Juue 24. Cultures on corn-meal saturated with 
beef broth, variously acidulated, inoculated with spores from ex¬ 
periment 15. Corn meal stirred in the beef broth until a thick 
batter was made, placed in tumblers, infected wdth spores from 
No. 15, and covered with glass. June 25, no grovdli of Sporotrichum. 
Experiment continued until the 27th with no change. 
Nos. 22 and 23. June 24. Cultures on corn meal saturated with 
extract of horse excrement acidulated with five per cent, and 
three per cent, solutions of tartaric acid respectively, and infected 
with spores from No. 15. Culture material placed in glass tumblers 
aud covered with glass. Experiment continued until June 27 
with no growth of Sporotrichum. 
The four following (Nos. 24-27) are unsuccessful test-tube cul¬ 
tures on corn-meal saturated with extract of horse excrement, va¬ 
riously acidulated, sterilized, and infected with fungus spores 
from experiments 15 and 77. 
Nos. 24 and 25. Juae 24. Media prepared as in experiments 16 
and 17, except that the extract was boiled a second time for half 
