44 
an hour, filtered through Jilter paper, and sterilized for half an 
hour at a temperature varying from 100 to 104 Cent. It was 
then acidulated with five per cent, of tartaric acid, mixed with 
corn-meal to form a stiff batter, placed in test-tubes and sterilized 
again for a half hour at 100° Cent, June 25, 11:45 a. m., fungus 
spores from No. 15 introduced with a sterilized platinum wire. July 
5, no growth in either tube. A second infection of fungus spores 
from No. 15 introduced into No. 25. July 11, no growth. 
Nos. 26 and 27, June 24. Like experiments 24 and 25, except 
that the fluid was acidulated with three per cent, of tartaric acid. 
Examined daily but no fungous growth seer. July 6, a second 
lot of spores w T as introduced from No. 77, a sterilized culture on 
bran saturated with beef broth and infected with spores from No. 
7. July 8, no fungous growth. July 11, no growth; experiments 
discontinued. 
No. 28, June 26. Culture on bread saturated with an extract 
of horse excrement, similar to experiments 16-23, in that the me¬ 
dium was not sterilized. Bread placed in a tin pan, soaked with 
the fluid, and infected with fungus spores from No. 15. June 30, 
a small patch of fungus, resembling Sporotrichum, growing on the 
crust. July 1, fungus of yesterday growing slowly. A second 
fungus, unlike the first, present on the bread. July 2, both fungi 
were growing freely. July 3, growdh still more prominent. July 
5, both fungi carefully compared with Sporotrichum globuliferum , 
and found to be very different. July 11, no Sporotrichum seeD. 
No. 29, June 29. Infection experiment with dead chinch-bug 
larvae and pupse from Blue Mound, Ill., treated with spores of 
the white muscardine from No, 15, and placed in a dry tumbler 
which was then covered with glass. July 2, a very slight myce¬ 
lial growth on one or two bugs. July 3, growth a little more 
prominent. July 5, a feeble growth on a few more insects. July 
24, no certaiu growth of Sporotrichum found on any of the dead 
bugs. 
No. 30 July 6. Infection experiment with live chinch bugs, 
consisting of larvae and pupae, from Mr. A. H. Moule of Green¬ 
ville. in Bond county. Bugs placed in fruit-jars with corn leaves 
for food, and infected with spores of the white fungus from Nos. 
15, 32, and 51. July 8, no fungus seen. Imagos more numerous. 
July 12, no fungous growth. Fresh food introduced. July 18, 
many bugs dead but no traces of Sporotrichum. Fresh food sup¬ 
plied. July 22, all dead, and no traces of the white fungus pres¬ 
ent. 
No. 31. July 10. Test-tube culture on corn meal and beef 
broth prepared as in No. 78, sterilized at a temperature varying 
from 100° to 106° Cent,, and infected with spores in the usual way. 
The tube was then placed in wet saud at a temperature of 
Cent. July 11, no fungous growth Temperature of saud 22 Cent. 
July 12, growing nicely. July 15, growth on the increase. July 
20, yellowish tinge; surface granular. July 24, spores ripe, and 
easily detached. 
