45 
Spores from this culture were used also to infect cultures Nos. 1 
and 26, Jan. 5 and March 12, (1 92). 
No. 32. June 9. Test tube culture on corn meal and beef broth 
prepared and sterilized as in No. 15. June 11, spores from ex¬ 
periment 14 introduced by means of a sterilized platinum wire. 
June 13, 8 a. m, mycelal threads of the whit-* fungus visible. 
Growing freely on the 14th; and still more so on the loth. June 16, 
surface of medium dry and cracked; fungus spreading and 
extending into crevices; heads of spores visible. June 17, 
little change; 18th, growth covering the entire surface of medium; 
slightly tinged with yellow. Two days later the growth had ex¬ 
tended well under the medium where this wag dry and separated 
from the sides of the tube. June 21, fresh growth in bottom of 
tube. Spores easily detached by jarring on the 22d, and readily 
loosened by shaking on the 24th. Spores ripe. 
No. 33. June 29. Infection experiment upon fifty cabbage 
worms (Pieris rapce) treated with spores from the preceding ex¬ 
periment (No. 32). Fifty cabbage worms were well dusted with 
fungus spores from No. 32, and placed in a common breeding-cage 
on damp sand, where they were supplied from day to day with 
fresh cabbage leaves for food. July 2, six worms were dead on 
the sand; and fourteen more had died on the following day. One 
showed an external fungous growth; and a microscopical examina¬ 
tion of some of the others showed that the body cavities were 
filled with mycelial threads. Dead worms placed on damp sand 
under a bell glass. Six more dead on the 4th; one covered with 
fungus. Some of those placed on sand the 3d, covered with Spo- 
rotrichum. July 5, all the worms dead except two, and these 
died the following day, the greater part having an external growth 
of the white fungus. July 6, fifteen of the worms used for in¬ 
fecting No. 35; other specimens preserved. 
A check consisting of a like number of worms, kept under pre¬ 
cisely similar conditions, was started the same day (June 29), 
and supplied with fresh food. It remained without loss; the 
worms matured and pupated, and butterflies emerged July 13. 
No. 34. July 6. Contagion experiment upon a lot of chinch- 
bugs exposed to cabbage worms covered with a growth of Sporo- 
trichum (which had not yet reached the fruiting stage) taken from 
the preceding experiment (No. 33). A large number of live larvae, 
pupm, and imagos of the chinch-bug, together with a number of 
dead bugs, were placed on growing corn and wheat under a bell-jar, 
together with fifteen cabbage worms dead with the white fungus 
from No. 33. July 11, no traces of fungous growth on the bugs; 
still no growth on the 15th; and no fungous developement on bugs 
the 24th. Experiment discontinued. 
No. 35. July 9. Culture on corn-meal and beef broth prepared 
as in No. 78, and infected with spores from No. 32. Meal and 
broth made into a thick batter, placed in a test-tube, sterilized for 
half an hour at a temperature of 100" to 106° Cent, and treated 
with fungus spores. The tube was then placed in an oven at a 
