48 
No. 42. June 30. Infection experiment from tomato worms 
{Protoparce celens), treated with spores of Sporotrichum from 
culture No. 41. Two tomato worms were infected by rubbing the 
fungus spores over the spiracles with a piece of cotton, and were 
then placed in a breeding-cage and supplied with food. No re¬ 
sults July 3, but the worms entered the earth to pupate the fol¬ 
lowing day. One adult emerged July 31 and was preserved, and 
the other came out August 6. No fungus seen. 
One worm was placed in a cage under similar conditions the 
same date (June 30) and kept as a check. The larva pupated 
July 4 and the adult emerged August 5. No Sporotrichum. 
Nos. 43 and 14. July 7. Culture experiments with a raw egg, 
the white being used in No. 43 and the yolk in No. 44. Placed 
sep irately in shallow dishes, treated with spores from culture 41 
and covered with glass. No growth; result negative. 
No. 45. July 7. A fruit-jar culture on corn-meal batter made 
with beef broth, the spores for which were taken from No. 41. 
The beef broth used in this experiment was made by cooking one 
pound of beef in a quart of water for three hours, after which 
the liquid was poured off, and used without further treatment. 
The jar was partly filled with corn-meal batter, and treated with 
fuugus spores July 8, introduced through the cap, altered to 
facilitate sterile culture (see Plate Y, Fig. 1). July 10, growth 
beginning; Ju'y 11, meal covered with a fine mycelial growth, 
which increased and spread daily. July 20, spore clusters of 
Sporotrichum showing quite distinctly. July 22, spores quite yel¬ 
lowish and loosened by jarring. July 7 24, fungus as before. 
No. 46. July 9. Infection experiment upon live chinch bugs 
treated with Sporotrichum spores from culture No. 41. About 
three hundred adult bugs of the second generation placed on grow¬ 
ing wheat, dusted freely with spores and covered with cheese cloth. 
Observed until July 23, at which time the bugs were all dead, but 
without evident signs of Sporotrichum infection. 
No. 47. July 9. Test-tube culture on corn meal saturated with 
beef broth; sterilized at a temperature varying from 100 to 106 3 
Cent, for half an hour, and infected with spores from culture 41. 
The tube was then heated to 60° Cent, for five minutes, allowed 
to cool, and kept at temperature of the air. A slight growth of 
the white fungus was noticed July 10; the growth slightly 
increased the 11th; still more abundant the 15th; spore clusters 
visible the 20th and slightly tinged with yellow\ Spores not easily’ 
detached by jarring, but seemingly ripe on the 24th. 
No. 48. July 27. Infection experiment upon Hemaris larvae 
inoculated with spores fron No. 47. Eight larva?, ranging from 
a few days’ old to nearly full grown, placed in a glass dish, 
dusted with Sporotrichum spores, and enclosed in a breeding- 
cage with bush honeysuckle leaves for food. One larva dead on 
August 2, and several more on the 3d. The dead larva were 
rigid and remained in natural attitudes on twigs of plant. August 
