49 
4, Sporotrichum appearing externally on the dead larva first 
noticed. Mycelial threads of the white muscardine found in body 
cavity of the others. August 5, another larva covered with the 
fungus, and the following day all except one showed an external 
growth of Sporotrichum August 10, spores were ripe on three 
lai vae, the growth maturing slowly on the others. Specimens of 
the same lot, kept in similar conditions as a check, had in the 
meantime continued without loss, and pupated in good condition. 
No. 49. . August 29. Infection experiment upon miscellaneous 
insects, principally grasshoppers, taken bv sweeping grass. The 
insects were dusted with spores from experiment 47 and 50, and 
placed in a breeding-cage with young corn plants for food. ’Sep¬ 
tember 1, several grasshoppers dead but no signs of the white 
fungus visible. Nearly all the insects had died bv the 5th \ on 
the 7th, a grasshopper with an external growth of Sporotrichum 
was removed, and still others on the 10th and 21st Specimens 
preserved. 
No. 50. July 9. Culture on corn meal and beef broth, in¬ 
fected^ with spores from culture No. 41. Broth prepared as in No. 
'78. Corn meal and broth mixed so as to form a thick batter, 
placed in a test-tube, sterilized for half an hour at a temperature 
varying from 100 c to 10(5° Cent, and treated wi h spores. July 
10, 5 p. m., a slight fungous growth apparent. July 1J, growing 
fieely. July 15, fungus spreading. July 20, yellowish tinge and 
granular appearance on surface. July 21, spares apparently ripe 
but not easily detached. 
No. ol. July 27. Infection experiment noon Datana larvm and 
fungus spores from the preceding culture, No. 5<». About sixty 
half-grown Datana larvae collected from apple-ti>es, tieated with 
spores, placed in a well-ventilated breeding cage, and supplied with 
apple leaves for food. July 81, two larvae dead <n earth in 
bottom of cage. August 1, fifteen more on earth either dead or 
dying. Mycelial threads abundant in body cavity of all those ex¬ 
amined. Ochers dead August 2, and those examined showed a 
gieateL abundance of mycelial threads in the body cavity, and in 
a more advanced stage of development than llnse examined the 
preceding day. A few of the dead larvae remained attached to the 
top of the cage for some time. August 3, most of the larvae dead 
and about half of; them hanging by their piolegs to the sides of 
the cage or to twigs, Mycelial growth conspicuous in body cavity, 
oome of the larvae first dead show an external growth of Sporo¬ 
trichum. . August 5, all the larvae dead except one. The white 
fungus v isible on about a dozen dead bodies. August 6 and 7, 
more larvae with an external fungous growth. August 11, more 
than thirty larvae imbedded in Sporotrichum removed and "placed 
m bottle. Several dead larvae covered with this same fungus left 
in the cage.* 
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—4 
