50 
July 27, an equal number of Datana larvae were placed in a 
breeding-cage under similar conditions and kept as a check. One 
had died on the 31st, but contained no trace of the white fungus 
either externally or internally. August 3, one more dead, but no 
fungus. August 5, remaining larvae all right. August 7, larvae 
almost full grown and in good condition. August 11, larvae all 
right, and some had entered ground to pupate. Sporotrichum did 
not appear at any time in this check. . . , XT 
No. 52. June 9. Culture experiment precisely similar to :so. 
41 The medium consisted of a batter of middlings mixed with beef 
broth, and was infected with spores, June 11, from culture No. 14. 
There was a slight growth of Sporotrichum on the surface ot the 
medium bv the 13th, which had increased but little by the follow¬ 
ing day. On the 15th, it was confined to two small patches. By 
the 16th, the medium had shrunken from the tube, and was quite 
dry on the surface. Fungous growth poor. A slight yellowish 
tint appeared the 17th; surface a little more granular on the 22d; 
on the 24th, no change. . j ,. , .., 
Nos. 53 and 54. June 9. Culture experiments identical with 
Nos. 39 and 40, except that the medium used was a batter of 
bran mixed with beef broth. The spores used for infection (June 
11) came from culture No. 14. Growth had started m both tubes 
by the morning of the 13th, and increased steadily, spreading over 
the surface of the medium. June 16, fungus penetrating the. air 
spaces throughout the greater part of the mass. Spores forming. 
June 17, surface granular,* with a yellowish tinge, which became 
more marked from day to day; otherwise no perceptible change 
up to June 20, when spores could with difficulty be shaken ott 
by heavy jarring. Spores ripe and easily detached June.22 to 24. 
No. 55. July 27. Infection experiment upon Hemaris larvae, 
precisely similar to No. 48, except that the spores used for infec¬ 
tion were derived from No. 14. Four larvae about two thirds 
grown were thoroughly treated with spores and placed in a bieed- 
lng-cage with food plant. August 1, several larvae formed cocoons. 
August 6, larvae dead in their cocoons, and one shows the presence 
of Sporotrichum. August 8, external fungous growth visible on 
all the larvae, and spores were foiming on one two days later. 
A check of a similar number kept under precisely the same 
conditions remained without loss and pupated in good condition. 
No. 56. December 21. Culture experiment identical with No. 
50, except that the fungus spores used came from No. 14. lhe 
corn-meal batter was placed in a test-tube and infected with 
spores from culture 14. A very slight fungous growth appealec 
December 22, wffiich grew slowly and spread over the surface or 
the medium by December 30. Spores were now forming, on the 
older part of the culture. Jauuary 4 (1892), fungus fruiting, sur¬ 
face with a yellowish tinge. Spores detached by vigorous jarring. 
January 11, growth covering the entire surface of medium, spoies 
ripe and easily detached. _ 
* The grannies of these notes are the heads of spores. 
